A0A898BDZ4 · A0A898BDZ4_9CAUD

Function

function

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. Once the capsid is packaged with the DNA, the terminase cleaves the viral genome concatemer and is substituted by the tail.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Nuclease activity probably requires 2 Mg2+ ions per subunit.

Features

Showing features for active site, binding site.

148250100150200250300350400450
TypeIDPosition(s)Description
Active site176For ATPase activity
Binding site293Mg2+ (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site439Mg2+ (UniProtKB | ChEBI); catalytic; for nuclease activity

GO annotations

AspectTerm
Cellular Componentviral terminase, large subunit
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Biological Processchromosome organization
Biological Processviral DNA genome packaging

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Terminase, large subunit
  • Alternative names
    • DNA-packaging protein gp2

Including 2 domains:

  • Recommended name
    Endonuclease
  • EC number
  • Recommended name
    ATPase
  • EC number

Organism names

Accessions

  • Primary accession
    A0A898BDZ4

Proteomes

Subcellular Location

Expression

Keywords

Interaction

Subunit

Interacts with the terminase small subunit; the active complex is composed of a monomer of the terminase large subunit and a nonamer ring of terminase small subunits. Interacts with the portal protein; this interaction allows the packaging of viral DNA.

Family & Domains

Features

Showing features for motif, domain.

TypeIDPosition(s)Description
Motif49-56Walker A motif
Motif171-176Walker B motif
Domain290-450Terminase large subunit gp17-like C-terminal

Domain

The ATPase region is in the N-terminus, whereas the nuclease region is in the C-terminus.

Sequence similarities

Belongs to the Lederbergvirus large terminase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    482
  • Mass (Da)
    54,620
  • Last updated
    2021-09-29 v1
  • Checksum
    F3E3FE6397D4F6FF
MDTQERLRNLVRELAERQKYFRMKQYTPYGWQEKFIAASSNCAQLLAMTGNRCGKTYTGAFIMACHLTGRYPEWWTGRKFDKPVNCWAAGISTDTTRDILQSELLGDWKNPEAFGTGMIPKEDIVKTERREGKPGCVQAVMVRHASGGLSSLIFKSYEMSQDKFMGTAIDVIWLDEECPKDIYTQCVTRTATTGGIVYLTFTPEHGLTEIVKDFLQDLKPGQFLIHASWEDAPHLSPEVKEQLLSVYSPAERRMRAEGIPMLGSGVVFPILEEKFVCEPFDIPDHFHRIIGIDLGFDHPNAIACVAWDAEKDKYYLYDERSESGETLGMHADAIYLKGGHQIPVVVPHDAFKHDGATSGRRFVDLLKDDHNLNVVYEPFSNPPGPDGKHGGNSVEFGVNWMLTRMENGDLKVFNTCTNFLKEMKMYHRKDGKIVDRNDDMISATRYALLMASRHARPGAVRNSGYYRSDTARLIPDWFGSIV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MW329986
EMBL· GenBank· DDBJ
QSH71736.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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