A0A898BDZ4 · A0A898BDZ4_9CAUD
- ProteinTerminase, large subunit
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids482 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. Once the capsid is packaged with the DNA, the terminase cleaves the viral genome concatemer and is substituted by the tail.
Cofactor
Note: Nuclease activity probably requires 2 Mg2+ ions per subunit.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | viral terminase, large subunit | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Biological Process | chromosome organization | |
Biological Process | viral DNA genome packaging |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameTerminase, large subunit
- Alternative names
Including 2 domains:
- Recommended nameEndonuclease
- EC number
- Recommended nameATPase
- EC number
Organism names
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Bruynoghevirus
Accessions
- Primary accessionA0A898BDZ4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Expression
Keywords
- Developmental stage
Interaction
Subunit
Interacts with the terminase small subunit; the active complex is composed of a monomer of the terminase large subunit and a nonamer ring of terminase small subunits. Interacts with the portal protein; this interaction allows the packaging of viral DNA.
Structure
Family & Domains
Features
Showing features for motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 49-56 | Walker A motif | ||||
Sequence: TGNRCGKT | ||||||
Motif | 171-176 | Walker B motif | ||||
Sequence: VIWLDE | ||||||
Domain | 290-450 | Terminase large subunit gp17-like C-terminal | ||||
Sequence: IGIDLGFDHPNAIACVAWDAEKDKYYLYDERSESGETLGMHADAIYLKGGHQIPVVVPHDAFKHDGATSGRRFVDLLKDDHNLNVVYEPFSNPPGPDGKHGGNSVEFGVNWMLTRMENGDLKVFNTCTNFLKEMKMYHRKDGKIVDRNDDMISATRYALLM |
Domain
The ATPase region is in the N-terminus, whereas the nuclease region is in the C-terminus.
Sequence similarities
Belongs to the Lederbergvirus large terminase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length482
- Mass (Da)54,620
- Last updated2021-09-29 v1
- ChecksumF3E3FE6397D4F6FF