A0A853MHX5 · A0A853MHX5_9CYAN

Function

function

The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
    EC:3.1.21.10 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ ion per subunit.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site9
Binding site9Mg2+ 1 (UniProtKB | ChEBI)
Active site76
Binding site76Mg2+ 2 (UniProtKB | ChEBI)
Active site148
Binding site148Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentHolliday junction resolvase complex
Molecular Functioncrossover junction DNA endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Crossover junction endodeoxyribonuclease RuvC
  • EC number
  • Alternative names
    • Holliday junction nuclease RuvC
    • Holliday junction resolvase RuvC

Gene names

    • Name
      ruvC
    • ORF names
      A9P98_14305

Organism names

Accessions

  • Primary accession
    A0A853MHX5

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.

Family & Domains

Sequence similarities

Belongs to the RuvC family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    166
  • Mass (Da)
    18,401
  • Last updated
    2021-09-29 v1
  • Checksum
    08C7A5DFAC94FA6C
MTRQILGLDPGLAIVGFGAISLEQRSAKLSDTTITMVDFGVIHTPSDMEITQRLCTLFDDLHTLLDQLQPDVVAIERLFFYRMANTILVAQARGVIMLVLGQRRLPYLEFAPPQIKQALTSYGKAEKIEVQEAVMRELDLEEIPQPDDAADALALALTAAFKLTDD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LYXA01000001
EMBL· GenBank· DDBJ
OBU78132.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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