A0A853MDB5 · A0A853MDB5_9CYAN
- Protein2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
- GenemenD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids578 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).
Catalytic activity
- 2-oxoglutarate + H+ + isochorismate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 1 thiamine pyrophosphate per subunit.
Pathway
Cofactor biosynthesis; phylloquinone biosynthesis.
Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 2/7.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | manganese ion binding | |
Molecular Function | thiamine pyrophosphate binding | |
Biological Process | menaquinone biosynthetic process | |
Biological Process | phylloquinone biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
- EC number
- Short namesSEPHCHC synthase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Nostocales > Aphanizomenonaceae > Cylindrospermopsis
Accessions
- Primary accessionA0A853MDB5
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-127 | Thiamine pyrophosphate enzyme N-terminal TPP-binding | ||||
Sequence: VVVSTLKHLGLDFAILCPGSRSTPLTLAFCQEGSDIESIAILDERSGAFFALGRAKATRKPVVLVCTSGTAGANFYPAVIEAKESCVPLLLLTADRPPELRDCHSGQTIDQVK | ||||||
Domain | 220-406 | Menaquinone biosynthesis protein MenD middle | ||||
Sequence: YERGIIIAGVAQPQDPEKYCQAISCLSRTLQWPVLAEGLSPVRNYADLCPYLISTYDLILRNPQLRQELTPQIIIQIGEMPTSKELRHWLVEVNAQRFVIDSSFENIDPLHGKTVHLRISVEDLDFGGVWDSMANLCLQQYLQQWCAIEAAVRKNIDDMFLKTEELTESKAAWLISQLLPANTPLFI |
Sequence similarities
Belongs to the TPP enzyme family. MenD subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length578
- Mass (Da)65,107
- Last updated2021-09-29 v1
- Checksum959B299949853BB0