A0A852MPM2 · A0A852MPM2_9PASS
- ProteinLanosterol 14-alpha demethylase
- GeneCyp39a1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids448 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
Catalytic activity
- 24,25-dihydrolanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H+This reaction proceeds in the forward direction.
CHEBI:28113 + 3 RHEA-COMP:11964 + 3 CHEBI:15379 = CHEBI:78904 + CHEBI:15740 + 3 RHEA-COMP:11965 + 4 CHEBI:15377 + 4 CHEBI:15378 - 24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxy-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- 32-hydroxy-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxo-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H+This reaction proceeds in the forward direction.
- 32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H+This reaction proceeds in the forward direction.
- 32-oxo-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H+This reaction proceeds in the forward direction.
- 32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H+This reaction proceeds in the forward direction.
- a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta14 steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H+This reaction proceeds in the forward direction.
- a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H+This reaction proceeds in the forward direction.
- a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
- lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H+This reaction proceeds in the forward direction.
Cofactor
Pathway
Lipid metabolism; bile acid biosynthesis.
Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 256 | substrate | |||
Binding site | 393 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxysterol 7-alpha-hydroxylase activity | |
Molecular Function | steroid 7-alpha-hydroxylase activity | |
Biological Process | bile acid biosynthetic process | |
Biological Process | cholesterol homeostasis | |
Biological Process | cholesterol metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLanosterol 14-alpha demethylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Telluraves > Australaves > Passeriformes > Sylvioidea > Timaliidae > Pteruthius
Accessions
- Primary accessionA0A852MPM2
Proteomes
Subcellular Location
Structure
Sequence
- Sequence statusFragment
- Length448
- Mass (Da)51,360
- Last updated2021-09-29 v1
- Checksum62E27AC64275EDF1
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-terminal residue | 1 | ||||
Non-terminal residue | 448 | ||||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
WEIY01000383 EMBL· GenBank· DDBJ | NXY07382.1 EMBL· GenBank· DDBJ | Genomic DNA |