A0A850W746 · A0A850W746_FREMA

Function

function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Probably binds two magnesium or manganese ions per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site, site.

TypeIDPosition(s)Description
Active site25
Active site64Proton donor/acceptor
Binding site64Mg2+ 1 (UniProtKB | ChEBI)
Binding site66Mg2+ 1 (UniProtKB | ChEBI)
Site66Transition state stabilizer
Site133Important for catalytic activity
Binding site158Mg2+ 1 (UniProtKB | ChEBI)
Active site159Proton acceptor
Binding site159Mg2+ 1 (UniProtKB | ChEBI)
Site159Interaction with DNA substrate

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular FunctionDNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Functiondouble-stranded DNA 3'-5' DNA exonuclease activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular Functionmetal ion binding
Molecular Functionphosphoric diester hydrolase activity
Biological Processbase-excision repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA repair nuclease/redox regulator APEX1
  • EC number
  • Short names
    APEN
    ; REF-1
  • Alternative names
    • APEX nuclease
    • Apurinic-apyrimidinic endonuclease 1
    • Redox factor-1
  • Cleaved into 1 chains

Gene names

    • Name
      Apex1
    • ORF names
      FREMAG_R06815

Organism names

  • Taxonomic identifier
  • Strain
    • B10K-DU-002-48
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Suliformes > Fregatidae > Fregata

Accessions

  • Primary accession
    A0A850W746

Proteomes

Subcellular Location

Nucleus
Cytoplasm
Mitochondrion

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain5-159Endonuclease/exonuclease/phosphatase

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    168
  • Mass (Da)
    19,158
  • Last updated
    2021-09-29 v1
  • Checksum
    198972E6B82FF80F
GEEEHDREGRVVTAEFPGLFVVAAYVPNAGRGLVRLEYRRRWDAAFRSYLARLDAQKPVALCGDLNVAHAEIDLRHPRANRRSAGFTVEEREGXLDAGFLDSFRHLYPTVPYAYTFWTYLGGARDRNVGWRLDYFVISRRLEGALCDSKIRSRVLGSDHCPITLYLAL

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue168

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WAAD01031973
EMBL· GenBank· DDBJ
NWH52286.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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