A0A850W746 · A0A850W746_FREMA
- ProteinDNA repair nuclease/redox regulator APEX1
- GeneApex1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids168 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Probably binds two magnesium or manganese ions per subunit.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 25 | |||||
Sequence: Y | ||||||
Active site | 64 | Proton donor/acceptor | ||||
Sequence: D | ||||||
Binding site | 64 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 66 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Site | 66 | Transition state stabilizer | ||||
Sequence: N | ||||||
Site | 133 | Important for catalytic activity | ||||
Sequence: D | ||||||
Binding site | 158 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 159 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 159 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 159 | Interaction with DNA substrate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | double-stranded DNA 3'-5' DNA exonuclease activity | |
Molecular Function | endonuclease activity | |
Molecular Function | lyase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoric diester hydrolase activity | |
Biological Process | base-excision repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA repair nuclease/redox regulator APEX1
- EC number
- Short namesAPEN ; REF-1
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Suliformes > Fregatidae > Fregata
Accessions
- Primary accessionA0A850W746
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-159 | Endonuclease/exonuclease/phosphatase | ||||
Sequence: HDREGRVVTAEFPGLFVVAAYVPNAGRGLVRLEYRRRWDAAFRSYLARLDAQKPVALCGDLNVAHAEIDLRHPRANRRSAGFTVEEREGXLDAGFLDSFRHLYPTVPYAYTFWTYLGGARDRNVGWRLDYFVISRRLEGALCDSKIRSRVLGSDH |
Sequence similarities
Belongs to the DNA repair enzymes AP/ExoA family.
Family and domain databases
Sequence
- Sequence statusFragment
- Length168
- Mass (Da)19,158
- Last updated2021-09-29 v1
- Checksum198972E6B82FF80F
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: G | ||||||
Non-terminal residue | 168 | |||||
Sequence: L |
Keywords
- Technical term