A0A845KWF7 · A0A845KWF7_9FIRM
- ProteinGlutamate--tRNA ligase
- GenegltX
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids487 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
Catalytic activity
- ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | glutamate-tRNA ligase activity | |
Molecular Function | tRNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | glutamyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamate--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Lachnospirales > Lachnospiraceae > Dorea
Accessions
- Primary accessionA0A845KWF7
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 3-325 | Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic | ||||
Sequence: KVRTRFAPSPTGRMHVGNLRTALYTYLIAKHEDGDFILRIEDTDQERFVEGALDIIYRTLKETGLVHDEGPDKDGGVGPYVQSERNAQGLYLKYAEQLIEQGDAYYCFCDKERLESLKSKVSDEDGGTEIVVYDKHCLHLSKEEIEANLAAGKPHVIRFNMPTEGTTTFHDEIYGDITVPNEELDDLILIKSDGYPTYNFANVVDDHLMGITHVVRGNEYLSSAPKYNKIYEAFGWDVPIYVHCPLITDENHKKLSKRCGHSSYEDLIEQGFVTEAVVNYVALLGWSPEGNQEIFSLEELVKAFDFHRMNKSPAVFDLGKLKW | ||||||
Motif | 10-20 | 'HIGH' region | ||||
Sequence: PSPTGRMHVGN | ||||||
Motif | 256-260 | 'KMSKS' region | ||||
Sequence: KLSKR | ||||||
Domain | 339-482 | Aminoacyl-tRNA synthetase class I anticodon-binding | ||||
Sequence: FYERAEGYIRKVITKDYDLKKIAALVKSRIEIFPDIAEQIDFFEAVPEYDTAMYCHKKMKTNEENSLEVLREVLPLLEAQEDYSNDALFALLKSYVDEKGVKVGYVMWPIRTAVSGKQSTPGGATEIMEILGKEESVKRIKDGI |
Sequence similarities
Family and domain databases
Sequence
- Sequence statusComplete
- Length487
- Mass (Da)55,513
- Last updated2021-09-29 v1
- Checksum53A3BA5EF8150E80