A0A841HAJ4 · A0A841HAJ4_HALSI

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site609ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      HNR49_000680

Organism names

Accessions

  • Primary accession
    A0A841HAJ4

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain20-646Aminoacyl-tRNA synthetase class Ia
Motif50-60'HIGH' region
Motif606-610'KMSKS' region
Domain702-850Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,070
  • Mass (Da)
    119,902
  • Last updated
    2021-09-29 v1
  • Checksum
    EABADEAC91ECBDC3
MSGFGDVPDQYDPAGVEERVFSYWEAVDAYEQTVDHRADAETFFFVDGPPYTSGAAHMGTTWNKTLKDAYIRYHRMQGYDVTDRPGYDMHGLPIETKVEEQLGFESKKDIQEFGEEAFIEECKRFADDNLDGLQSDFQSFGVWMDWDNPYKTVDPSYMEAAWWAFSEVHDNGLVERGQRSISQCPRCETAIANNEVEYEDVTDPSIYVRFDLDDREGSLVVWTTTPWTIPANQFVAVDEDGDYQQVRATTPDGETDVLYVASECVEDVLSAGGYSDHEIVADHSGSDLIGWSYTPPLADAVPANPTDADGTHEVYHGDWVEADRTGLVHSAPGHGEEDFERGAELDLPVFCPVGEDGVYTDAGGKYAGAFVRDANDDIIADLEARDAMLASQTVEHSYGHCWRCDTGIIQIVTDQWFITITDVKDELLANMDTSEWHPEWARDNRFRDFVENAPDWNVSRQRYWGIPVPIWTPEDWSGDAEDVLVVGTREELAALADQDVDPASVDLHKPTVDDITITEDGTEYTRVPDVFDVWLDSSVASWGTLNYPEQEDDFDELWPADLIMEAHDQTRGWFWSQLGMGTAALGDVPYEEVLMHGYANMPDGRGMSKSKGITIEPNEVIDEYGADPMRMFLLSVTPQGDDMSFSWDETENMQRDLNILWNVFRFPRPYMALDDFDANVPAAFGGDDAGVGIADADLETVDEWLLSRLQHVKADATAHWEDFEQHRALDAVLEFVVEDLSRYYVQVVRERMWEDDASASKTAAYATMQRVLLEVVALLAPYAPFVTEELYSHLTGDRGYDTVHMADWPTVEESLRAPALEADVAVLRAAEEAGSHARQQAGRKLRWPVTRVVVDAAEDSVVDALDAHGDLLADRLNTRAIEVVEPGAAWDELAYSARADMSELGPAFGDDAGAVMNALNDARVTDRDIDALAEQVAADLGRDDIELTTEMVEFVEETPEHVAGAAFETETAAGTVYVDTELNEDVESEGYAREVVRRVQEMRKEMDLAMDAEIRLDVLVFDERVGELVARHEPLITAETRARELGEVEDGHREEWDVEGTTMILEVEAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACHGX010000001
EMBL· GenBank· DDBJ
MBB6089342.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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