A0A829R5X5 · A0A829R5X5_LISGR
- ProteinMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- GenetrmFO
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids434 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.
Catalytic activity
- uridine54 in tRNA + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADH + H+ = 5-methyluridine54 in tRNA + (6S)-5,6,7,8-tetrahydrofolate + NAD+
RHEA-COMP:10193 CHEBI:65315 Position: 54+ CHEBI:15636 + CHEBI:57945 + CHEBI:15378 = RHEA-COMP:10167 CHEBI:74447 Position: 54+ CHEBI:57453 + CHEBI:57540
Cofactor
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | tRNA (uracil(54)-C5)-methyltransferase activity, 5,10-methylenetetrahydrofolate-dependent | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Listeriaceae > Listeria
Accessions
- Primary accessionA0A829R5X5
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-366 | MnmG N-terminal | ||||
Sequence: KVNVIGAGLAGSEAAWQLAQRGIQVALYEMRPVKQTPAHHTDKFAELVCTNSLRANSLTNAVGLIKEEMRILDSIIINAADRASVPAGGALAVDRHEFSGYITEQVKQHPFVTVYQEEVTAIPEGPTIIATGPLTSPELAESIKALTGSSYLYFYDAAAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEREFFAFYRALVEAETAELKDFEKEIYFEGCMPIEVMAKRGMKTLLFGPMKPVGLENPHTGRRPYAVLQLRQDDAAGTLYNMVGFQTHLKWGEQKRVFQMIPGLENAEIVRYGVMHRNTFINSPEILLPTYQLKAREDLFFAGQMTGVEGYVESAASGLAAGINAAKYVSGE |
Sequence similarities
Belongs to the MnmG family. TrmFO subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length434
- Mass (Da)48,241
- Last updated2021-09-29 v1
- ChecksumA0A2647F1DD2C018