A0A804HJ11 · A0A804HJ11_HUMAN
- ProteinUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
- GeneDPAGT1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids379 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.
Catalytic activity
- a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMPThis reaction proceeds in the forward direction.
a di-trans,poly-cis-dolichyl phosphate RHEA-COMP:19498 + CHEBI:57705 = an N-acetyl-α-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19507 + CHEBI:57865
Cofactor
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | glycosyltransferase activity | |
Molecular Function | UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity | |
Biological Process | dolichol-linked oligosaccharide biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionA0A804HJ11
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 12-32 | Helical | |||
Transmembrane | 63-85 | Helical | |||
Transmembrane | 97-118 | Helical | |||
Transmembrane | 138-159 | Helical | |||
Transmembrane | 166-182 | Helical | |||
Transmembrane | 194-212 | Helical | |||
Transmembrane | 224-242 | Helical | |||
Transmembrane | 248-268 | Helical | |||
Transmembrane | 350-372 | Helical | |||
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 324 variants from UniProt as well as other sources including ClinVar and dbSNP.
Genetic variation databases
PTM/Processing
Expression
Gene expression databases
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length379
- Mass (Da)42,814
- Last updated2021-09-29 v1
- ChecksumC75E60A16DCA1F0B
Computationally mapped potential isoform sequences
There are 15 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q9H3H5 | GPT_HUMAN | DPAGT1 | 408 | ||
F8WE55 | F8WE55_HUMAN | DPAGT1 | 95 | ||
F8W681 | F8W681_HUMAN | DPAGT1 | 57 | ||
A0A1B0GV58 | A0A1B0GV58_HUMAN | DPAGT1 | 77 | ||
A0A1W2PPC6 | A0A1W2PPC6_HUMAN | DPAGT1 | 377 | ||
A0A1W2PQH0 | A0A1W2PQH0_HUMAN | DPAGT1 | 173 | ||
A0A1W2PQD1 | A0A1W2PQD1_HUMAN | DPAGT1 | 69 | ||
A0A1W2PRR6 | A0A1W2PRR6_HUMAN | DPAGT1 | 102 | ||
A0A804HI18 | A0A804HI18_HUMAN | DPAGT1 | 242 | ||
A0A804HHX1 | A0A804HHX1_HUMAN | DPAGT1 | 59 | ||
A0A804HIP7 | A0A804HIP7_HUMAN | DPAGT1 | 67 | ||
A0A804HJ22 | A0A804HJ22_HUMAN | DPAGT1 | 78 | ||
A0A804HL46 | A0A804HL46_HUMAN | DPAGT1 | 276 | ||
A0A804HKZ3 | A0A804HKZ3_HUMAN | DPAGT1 | 313 | ||
H7C2L6 | H7C2L6_HUMAN | DPAGT1 | 226 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP003391 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |