A0A803KKY0 · A0A803KKY0_PANTR

Function

function

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site46Crucial for catalytic activity
Binding site112-125acetyl-CoA (UniProtKB | ChEBI)
Binding site148-157acetyl-CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentaxon
Cellular Componentclathrin-coated pit
Cellular Componentcytoplasm
Cellular Componentfocal adhesion
Cellular Componentmicrotubule
Cellular Componentspindle
Molecular Functiontubulin N-acetyltransferase activity
Biological Processneuron development
Biological Processregulation of microtubule cytoskeleton organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-tubulin N-acetyltransferase 1
  • EC number
  • Short names
    Alpha-TAT
    ; Alpha-TAT1
    ; TAT
  • Alternative names
    • Acetyltransferase mec-17 homolog

Gene names

    • Name
      ATAT1
    • Synonyms
      MEC17
    • ORF names
      CK820_G0006761

Organism names

  • Taxonomic identifier
  • Strain
    • Yerkes chimp pedigree #C0471
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Pan

Accessions

  • Primary accession
    A0A803KKY0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain, modified residue.

TypeIDPosition(s)Description
Signal1-15
ChainPRO_503113283416-411Alpha-tubulin N-acetyltransferase 1
Modified residue44N6-acetyllysine; by autocatalysis
Modified residue134N6-acetyllysine; by autocatalysis

Post-translational modification

Autoacetylation strongly increases tubulin acetylation.

Keywords

Interaction

Subunit

Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain3-178N-acetyltransferase
Region184-227Disordered
Compositional bias212-226Basic and acidic residues
Region240-272Disordered
Compositional bias243-258Pro residues
Region294-390Disordered
Compositional bias325-344Polar residues

Sequence similarities

Belongs to the acetyltransferase ATAT1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    411
  • Mass (Da)
    45,772
  • Last updated
    2021-06-02 v1
  • Checksum
    6326A4295293C993
MWLTWPFCFLTITLREEGVCHLESVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPTAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTRGTPPGLVAQSCCYSRHGGVNSSSPNTGNQDSKQGEQETKNRSASEEQALSQDGSGEKPMHTAPPQAPAPAPPAQSWTVGGDILNARFIRNLQERRSTRPW

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias212-226Basic and acidic residues
Compositional bias243-258Pro residues
Compositional bias325-344Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NBAG03000221
EMBL· GenBank· DDBJ
PNI76367.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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