A0A7Z3DWP0 · A0A7Z3DWP0_9RICK

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

110911002003004005006007008009001,000
TypeIDPosition(s)Description
Binding site653ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      GOY13_00300

Organism names

Accessions

  • Primary accession
    A0A7Z3DWP0

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain22-682Aminoacyl-tRNA synthetase class Ia
Motif50-60'HIGH' region
Motif650-654'KMSKS' region
Domain739-880Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,091
  • Mass (Da)
    127,162
  • Last updated
    2021-06-02 v1
  • Checksum
    F740AFA109DD45B7
MKHYPSTTSDPDFSLLEREIIKFEKENKIFELSVKKRSKDNCFTFYDGPPFANGLPHYGHLLTGFIKDTFARYQTMLQKRVERRFGWDCHGLPAEMSAEKDLGISGRTEIERFGIKKFNDHCRASVMKFTLEWERYVNRQARWVDFHNCYRTMDKSFMESVMWAFKQLYNMGLVYESVRVVPYSWACETPLSNFETKLDNSYRKRTSKAVIVAFELLKNPQQFRQKCKLLAWTTTPWTLPSNLALAIGKDIKYCAVLTNPLVSLQHLKSKSIPESRYQCARTAESVGIAVEVNRRNLVGSEIYIFAESYLEKFISYCKQNGIEYENCNIRLKANDLIGLCYKPLFNYFKDIKNAFQVFVADYVTEEDGTGIVHAAPGFGEEDFYLCQSHSIPAVCPIDNSGKFTIEVPDLAGVHVFDANDLIIKKLKEQGNWFKTEQYIHNYPHCWRTDTPLIYRAMPSWYIAVTKFKEKMIELNKRVNWIPGHIRDGQFGKWLEGVHDWSISRNRFWGTPIPIWKSDDARYPRVDVYGSIAELEQDFNVKIGDLHRPFVDNLTRPNPDDPTKKSIMRRVSDVFDCWFESGSMPFAQVHYPFENQKWFEDNFPADFITEYMAQTRGWFYTLFVLSTALFNSEPFKNCICHGVILDIKGQKLSKRLNNYVDPMEFFDKYGSDALRFLMLSSSIVCGGSLLLDKEGKSIRDVLRNVIKPIWNGYHFFTMYANADEIKAKVCKDYQSIIDCYMIAKCFEAVEKIQTSMSGYDSQQACKVIVDFFEVLNNWYIRSNRKRFWKSGLDQDKIDAYNVLYTVFYYILKAAAPLLPLITEAIWRGLEYGEISVHLTDFPQLEKYDSRFIAKMDLVRKACNAALSIRNTFNIRIRQPLSGMVIYHKFFSGFLEDDYQKMIKAEVNVKNLKLVDKLEGVASLELKLNFSLLGKKVPNKIKKLVQYVKKGKWRQIADGQIFLGDRSESYIVQKGEYKLLLKANSEYSAVFDNNEGIVTLNTEMDDDLILEGIARDVIRLIQETRKQANFHLSDKIMVMIKTENKKIREAVSEWSKYIKEQTLALSLEVSVKIKDDFYFKEYQDLIIGVRLNY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP046579
EMBL· GenBank· DDBJ
QKX01422.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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