A0A7Z2YD84 · A0A7Z2YD84_9VIBR

  • Protein
    Ribonuclease E
  • Gene
    rne
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

1987100200300400500600700800900
TypeIDPosition(s)Description
Binding site302Mg2+ (UniProtKB | ChEBI); catalytic
Binding site345Mg2+ (UniProtKB | ChEBI); catalytic
Binding site403Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site406Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      GT360_04765

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • HN897
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Vibrionales > Vibrionaceae > Vibrio

Accessions

  • Primary accession
    A0A7Z2YD84

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-119S1 motif
Region403-406Required for zinc-mediated homotetramerization and catalytic activity
Region541-562Disordered
Compositional bias578-694Basic and acidic residues
Region578-833Disordered
Compositional bias726-747Basic and acidic residues
Compositional bias764-788Basic residues
Region895-944Disordered
Region965-987Disordered
Compositional bias970-987Polar residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    987
  • Mass (Da)
    110,041
  • Last updated
    2021-06-02 v1
  • Checksum
    55C4D705B8A61BE4
MKRMLINATQKEELRVALVDGQKLFDLDIESPGHESKKANIYKGRITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPQGYSYQGRPSIKEVLTEGQEVIVQVEKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERTQLKAALSTLELPQGMGLIVRTAGVGKSAEELEWDLNVLLNHWGAIKQASDSNEAPFLIHQESNVIVRAIRDYLRRDIGEILIDSNTIYERAVEHIRLVRPDFINRVKKYDGEVPLFSHYQIESQIESAFQREVRLPSGGSIVIDPTEALTSIDINSARATKGGDIEETALNTNLEAADEIARQLRLRDLGGLVVIDFIDMTPVRHQREVENRLRDAVRLDRARVQIGRISRFGLLEMSRQRLSPSLAEASHHICPRCTGTGVVRDNESLALSVLRLIEEEALKDNTSQVLAVVPVSIASYLLNEKRRSVNHIERNQEVKITIVPNSDMETPHFEVIRVRDGEEQNILSYLVPKKLEAMKEAEARDVNETETKVRKVEEPVLKGFAAPEKVAPAPVVKPQAESPAKKEEKQGSGLARFFKAITSFLFGSTEKEEEKKEETQEKPRSNNRQRRNNNRNDQRRRNNNRDKNKRKPTRDEATSEQQDKGTKPTEKKQTRKPRQDRRNKRDRDEASKPSKVEEQGLKIAEEAQSEKPEQTRRDEKSTRVKERRQRRKLNKSVRVKDQVEQQATPAVDEAETQQVPLERKAEKAKAKPAKADDAVTNGEESQNGEKAPKGRRNRRSPRHLRASGQRRRRGRDRRPNPFRLRAGGVASPEMSMGKVMPKYDLPKPGSRKPRKSQGKQTSILSGVARPEASMAKVIIMRAAEVAPVVAKAPKQAVEAPKAAKPAPMIVPSVVNAVIPKLASTPAVTPAPAKTTEAKVAPAVNKPVTAPKATKPASKPVVKPKGHASAAMAKAAGPQELRTIEVNAAPFRTERFVGKGAGSQVASNQATAGMTKPSY

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias578-694Basic and acidic residues
Compositional bias726-747Basic and acidic residues
Compositional bias764-788Basic residues
Compositional bias970-987Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP047475
EMBL· GenBank· DDBJ
QIA62869.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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