A0A7X2VIH2 · A0A7X2VIH2_9STRE

Function

function

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site32ATP (UniProtKB | ChEBI)
Binding site32CTP (UniProtKB | ChEBI)
Binding site35ATP (UniProtKB | ChEBI)
Binding site35CTP (UniProtKB | ChEBI)
Binding site45Mg2+ (UniProtKB | ChEBI)
Binding site47Mg2+ (UniProtKB | ChEBI)
Binding site116ATP (UniProtKB | ChEBI)
Binding site116CTP (UniProtKB | ChEBI)
Binding site159ATP (UniProtKB | ChEBI)
Binding site159CTP (UniProtKB | ChEBI)
Binding site162ATP (UniProtKB | ChEBI)
Binding site162CTP (UniProtKB | ChEBI)
Binding site165ATP (UniProtKB | ChEBI)
Binding site165CTP (UniProtKB | ChEBI)
Binding site168ATP (UniProtKB | ChEBI)
Binding site168CTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular Functionmagnesium ion binding
Molecular FunctiontRNA binding
Biological ProcessRNA repair
Biological ProcesstRNA 3'-terminal CCA addition

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CCA-adding enzyme
  • EC number
  • Alternative names
    • CCA tRNA nucleotidyltransferase
    • tRNA CCA-pyrophosphorylase
    • tRNA adenylyl-/cytidylyl- transferase
    • tRNA nucleotidyltransferase
    • tRNA-NT

Gene names

    • Name
      cca
    • ORF names
      GMC24_01880

Organism names

  • Taxonomic identifier
  • Strain
    • BIOML-A1
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus

Accessions

  • Primary accession
    A0A7X2VIH2

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain27-147Poly A polymerase head
Domain174-231tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding
Domain250-394CCA-adding enzyme C-terminal

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    406
  • Mass (Da)
    46,562
  • Last updated
    2021-06-02 v1
  • Checksum
    60E4599290EA629E
MRLKKLPSEFQEALPILEKIRKAGYEAYFVGGSVRDAILGRPIHDVDIASSSYPEETKAIFERTIDVGIEHGTVLVLENHQEYEITTFRTEDVYVDFRRPSAVNFVRSLEEDLKRRDFTVNAFALSEEGEIIDLFKGLEDLENRILRAVGVATERFNEDALRIMRGFRFQASLGFDLEEATFKAMTECAPLLEKISVERVFIELDKLLLAPFWRKGLEGVIASQSYQYLPLLQNSQSSLEKLFQLSMDYTFTSSEQAWAALLLTLDENKVSAFFKKWKTSRDFAKKVEQLVEIYRLREKASLNRRDVYRYDRSLLLSAEELRQAHGLPVDFQVIEEMYDSLAIHDKHEIVVNGGMLMKEYGLKPGPGLGQVLSAIEWAIVDGELENDKQAIGDFLSRYLEVKKGEA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WMXI01000001
EMBL· GenBank· DDBJ
MTQ41456.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp