A0A7X2VIH2 · A0A7X2VIH2_9STRE
- ProteinCCA-adding enzyme
- Genecca
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids406 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.
Miscellaneous
A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
Catalytic activity
- a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
- a tRNA with a 3' CCA end + ATP + 2 CTP = a tRNA with a 3' CCACCA end + 3 diphosphate
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 32 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 32 | CTP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 35 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 35 | CTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 45 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 47 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 116 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 116 | CTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 159 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 159 | CTP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 162 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 162 | CTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 165 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 165 | CTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 168 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 168 | CTP (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | CCA tRNA nucleotidyltransferase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | tRNA binding | |
Biological Process | RNA repair | |
Biological Process | tRNA 3'-terminal CCA addition |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCCA-adding enzyme
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus
Accessions
- Primary accessionA0A7X2VIH2
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 27-147 | Poly A polymerase head | ||||
Sequence: AYFVGGSVRDAILGRPIHDVDIASSSYPEETKAIFERTIDVGIEHGTVLVLENHQEYEITTFRTEDVYVDFRRPSAVNFVRSLEEDLKRRDFTVNAFALSEEGEIIDLFKGLEDLENRILR | ||||||
Domain | 174-231 | tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding | ||||
Sequence: GFDLEEATFKAMTECAPLLEKISVERVFIELDKLLLAPFWRKGLEGVIASQSYQYLPL | ||||||
Domain | 250-394 | CCA-adding enzyme C-terminal | ||||
Sequence: TFTSSEQAWAALLLTLDENKVSAFFKKWKTSRDFAKKVEQLVEIYRLREKASLNRRDVYRYDRSLLLSAEELRQAHGLPVDFQVIEEMYDSLAIHDKHEIVVNGGMLMKEYGLKPGPGLGQVLSAIEWAIVDGELENDKQAIGDF |
Sequence similarities
Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length406
- Mass (Da)46,562
- Last updated2021-06-02 v1
- Checksum60E4599290EA629E