A0A7W9IBW1 · A0A7W9IBW1_9ACTN
- ProteinL-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
- GenemshC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids407 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.
Catalytic activity
- 1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside + L-cysteine + ATP = 1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside + AMP + diphosphate + H+
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 43 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 43-46 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | ||||
Sequence: CGIT | ||||||
Binding site | 58 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 81-83 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | ||||
Sequence: NAT | ||||||
Binding site | 223 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 227 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 245-247 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | ||||
Sequence: GSD | ||||||
Binding site | 252 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 279 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | ||||
Sequence: V |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | cysteine-glucosaminylinositol ligase activity | |
Molecular Function | cysteine-tRNA ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | cysteinyl-tRNA aminoacylation | |
Biological Process | mycothiol biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
- EC number
- Short namesL-Cys:GlcN-Ins ligase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Streptosporangiales > Streptosporangiaceae > Streptosporangium
Accessions
- Primary accessionA0A7W9IBW1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for region, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-21 | Disordered | ||||
Sequence: MRSWSAPKVPRLPGTGAPPRL | ||||||
Domain | 38-331 | tRNA synthetases class I catalytic | ||||
Sequence: ARMYVCGITPYDATHLGHANTYLAFDLVNRAWRDAGHEVHFTQNATDVDDPLLKRAEETGVDWQELAEREIELFRTDMEALRIIPPREYVGVTEAIDQVAALVVLLRDKGATYDLDGDVYFDVAAAPKFGAVSGYSEEQMIALSGERGGDPDRPGKKHPLDWLLWRAERPGEPSWASPLGRGRPGWHIECTAIALANLSSGFDVAGGGSDLIFPHHECGAHEGHVACGEWPFAKAYVHAGMVALDGEKMSKSKGNLVFVSRLRRQSDPMAIRLALLAHHYRSDWEWTPDQLASA | ||||||
Motif | 45-55 | 'HIGH' region | ||||
Sequence: ITPYDATHLGH | ||||||
Motif | 183-188 | 'ERGGDP' region | ||||
Sequence: ERGGDP | ||||||
Motif | 285-289 | 'KMSKS' region | ||||
Sequence: KMSKS |
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length407
- Mass (Da)44,225
- Last updated2021-06-02 v1
- Checksum2A3CF0B47BF9D162
Keywords
- Technical term