A0A7W7HVD7 · A0A7W7HVD7_9ACTN
- ProteinAcyl transferase domain-containing protein/dTDP-4-dehydrorhamnose reductase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids5109 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Catalytic activity
- (2E)-butenoyl-[ACP] + H+ + NADPH = butanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-decenoyl-[ACP] + H+ + NADPH = decanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-dodecenoyl-[ACP] + H+ + NADPH = dodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexadecenoyl-[ACP] + H+ + NADPH = hexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexenoyl-[ACP] + H+ + NADPH = hexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-octadecenoyl-[ACP] + H+ + NADPH = NADP+ + octadecanoyl-[ACP]This reaction proceeds in the forward direction.
- (2E)-octenoyl-[ACP] + H+ + NADPH = NADP+ + octanoyl-[ACP]This reaction proceeds in the forward direction.
- (2E)-tetradecenoyl-[ACP] + H+ + NADPH = NADP+ + tetradecanoyl-[ACP]This reaction proceeds in the forward direction.
- (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- 3-oxobutanoyl-[ACP] + H+ + NADPH = (3R)-hydroxybutanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxodecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxododecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxotetradecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxytetradecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- H+ + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + malonyl-[ACP] + tetradecanoyl-[ACP] = 3-oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- acetyl-[ACP] + H+ + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- butanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- decanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxododecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- dodecanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxotetradecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
Pathway
Lipid metabolism.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | lyase activity | |
Molecular Function | oxidoreductase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | macrolide biosynthetic process | |
Biological Process | toxin biosynthetic process |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Submitted names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micromonosporales > Micromonosporaceae > Actinoplanes
Accessions
- Primary accessionA0A7W7HVD7
Proteomes
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 33-451 | Ketosynthase family 3 (KS3) | ||||
Sequence: QEPIAIVGMACRLPGGVHGPDALWRLVDEGRDGITPFPDDRGWDVAALVGGGGSATGEGGFIAAGDFDAAFFGISPREAVAMDPQQRLLLETSWEAFEHAGIDPASLRGTRTGVFTGASGVDYIGVVMGSREDAEGHATTGLTASVISGRVSYALGLEGPALTVDTACSSSLVALHLAAQALRNGECSLALAGGVTVMATPMGFSGFTRQGGLAPDGRCKAFADAADGTGWAEGVGVLVVEKLSDARRNGHNVLAVVRGSAVNSDGASNGLTAPNGPSQQRVIRQALASAGLAARDVDVVEAHGTGTTLGDPIEAQALLATYGQDRATPLYLGSVKSNIGHTQAAAGVAGVIKMVMAMRHRTVPRTLHVDAPTSHVDWEAGAVRLLTEPLPWPAAERPARAGVSSFGISGTNAHVILEQ | ||||||
Domain | 1504-1579 | Carrier | ||||
Sequence: AFLLGLVRTEAAAVLGHAAAAAVDARREFRDLGFDSLTAVELRNRLAAVSGLRLPATMVFDYPTPEALAGFLLGEL | ||||||
Domain | 1599-2023 | Ketosynthase family 3 (KS3) | ||||
Sequence: DDPIVIVGMACRLPGGVTSPDELWDLVAGGRDGITGFPADRGWDLATLRDGGDGGRGRSATGHGGFLERVADFDAGFFGISPREALAMDPQQRLLLETSWEAFEHAGISPAGLRGSQTGVFAGAGGQDYTQLVMNSREDVEGHASTGLAASVVSGRVSYVLGLEGPAVTVDTACSSSLVALHLAAQALRAGECDLALAAGVTVMSTPMGFSGFTKQGGLAEDGRCKAFADAADGTGWAEGVGVLVVERLSDAIANGHEILAVVRGSAVNSDGASNGLTAPNGPSQQRVIRRALHAARLAPGDVDVVEGHGTGTTLGDPIEAQALLATYGRDREVPLLLGALKSNIGHTQAASGVAGVIKMVMAMRHGVVPRTLHVDRPSTHVDWSAGAVRLVTEPLPWPVVDRPWRAGVSSFGISGTNAHVIIEQ | ||||||
Domain | 2942-3017 | Carrier | ||||
Sequence: ALVLDLVRAEAAAVLAHGSPEAVGPRAEFRDLGFDSLTAVELRNRLSAVTGLRLSATMVYDYPTPTALAGYLLAEI | ||||||
Domain | 3036-3460 | Ketosynthase family 3 (KS3) | ||||
Sequence: ADPIVIVGMACRLPGGVQSPDDLWRLVAGERDGISAFPTDRGWDLHKLAAGGADGQGRSASLEGGFLSGLADFDAAFFGISPREAMAMDPQQRLLLETSWEAFESAGIDPATLAGSPTGVFMGTNGLDYSTLIAQSREDVGSLSGTGLASSVISGRLSYNFGLEGPSFTVDTACSSSLVALHSAVAALRNSECSLALAGGVTVMATPVSFTGFSVDGGLSADGRCKSYSDAADGTSWAEGVGVLVVERQSDAIANGHEILAVVRGTAVNSDGASNGITAPNGPSQQRVIRQALASAGLTFADVDVVEGHGTGTPLGDPIEAQALLATYGRDRDVPLLLGSIKSNIGHTQAASGVAGIIKMVMAMRHALVPRSLHLAEPSSHVDWQAGNIRLLREPAAWPHTDRPWRAGVSSFGISGTNTHVILEQ | ||||||
Region | 3876-3997 | N-terminal hotdog fold | ||||
Sequence: HPLLGAAMTLADTGEVVFTGLLSLRVHPWLADHRVAGQVTLPGSAWLELAVRAGDQVGAERVQDLELHAPLAIGGSAALQVRVGAPDASGVRTVSMHSRPENGDDPWVLHARGSLATGRDRI | ||||||
Domain | 3876-4178 | PKS/mFAS DH | ||||
Sequence: HPLLGAAMTLADTGEVVFTGLLSLRVHPWLADHRVAGQVTLPGSAWLELAVRAGDQVGAERVQDLELHAPLAIGGSAALQVRVGAPDASGVRTVSMHSRPENGDDPWVLHARGSLATGRDRIDFEVPAWPPPYATEADLDGFHEVHGHGPAFTGLRAVWLHADEAYAEVALPEPVAGDAQYFGVHPALLASITHVTGLLGLDDELQPGRWGGVTLHAAGATTLRARIVRRGADRVEIAAVDPTGAPVLSVRELTLHRPRTVAAAARLPLFRMDWPAVEAPTPSSVRHAVLGDSVAELPSDVEV | ||||||
Region | 4009-4178 | C-terminal hotdog fold | ||||
Sequence: ATEADLDGFHEVHGHGPAFTGLRAVWLHADEAYAEVALPEPVAGDAQYFGVHPALLASITHVTGLLGLDDELQPGRWGGVTLHAAGATTLRARIVRRGADRVEIAAVDPTGAPVLSVRELTLHRPRTVAAAARLPLFRMDWPAVEAPTPSSVRHAVLGDSVAELPSDVEV | ||||||
Domain | 4738-4813 | Carrier | ||||
Sequence: RMLVDIVRTEAAAVLGHATSDAVDAERNFYELGFDSLTAVELRNRLSVATGLRLPATVIFDSKTPAELAGWIRAEL |
Family and domain databases
Sequence
- Sequence statusComplete
- Length5,109
- Mass (Da)530,338
- Last updated2021-06-02 v1
- Checksum1E32BF5751BC3E0C
Keywords
- Technical term