A0A7W5FZA4 · A0A7W5FZA4_9HYPH

  • Protein
    Bifunctional enzyme IspD/IspF
  • Gene
    ispDF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site21Transition state stabilizer
Site30Transition state stabilizer
Site164Positions MEP for the nucleophilic attack
Site221Positions MEP for the nucleophilic attack
Binding site250a divalent metal cation (UniProtKB | ChEBI)
Binding site250-2524-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site252a divalent metal cation (UniProtKB | ChEBI)
Site276Transition state stabilizer
Binding site276-2774-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site284a divalent metal cation (UniProtKB | ChEBI)
Binding site298-3004-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site374-3774-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site375Transition state stabilizer
Binding site3814-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site3844-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional enzyme IspD/IspF

Including 2 domains:

  • Recommended name
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • EC number
  • Alternative names
    • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    • MEP cytidylyltransferase
      (MCT
      )
  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispDF
    • ORF names
      FHS26_001702

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • CECT 4113
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Rhizobium

Accessions

  • Primary accession
    A0A7W5FZA4

Proteomes

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-2432-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Domain244-3962-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region244-4022-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
Belongs to the IspF family.
In the C-terminal section; belongs to the IspF family.
In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    402
  • Mass (Da)
    42,840
  • Last updated
    2021-06-02 v1
  • Checksum
    1CED2CC30FD48260
MPSKQPISAGIVIVAAGRGERAGSSKEGPKQYRMIGGKPVIVHTLENFMTWELATQIVVVIHPDDEALFARALRHIISATPIETVHGGATRQQSVLAGLKYLKDKHVSHVLIHDAVRPFFDHVLLDRIAESLGDGAPAVLPAMPVTDTLKRTDSAGTVLATVSRESLYAAQTPQSFAFETILDAHEKAAASGRSDFTDDASIAEWLGIPVTIVEGTADNVKLTVRSDIAKADDKLSASLLPDVRTGNGYDVHQLVAGDGVTLCGVFIPHDQKLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAASKIFIEHAARIVRERGGTIMNADVSLIAEAPKIGPHRDAMRAKLSDYLGIDIERCSVKATTNETIGFVGRREGIAAIATATVVYRGRK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACHXH010000005
EMBL· GenBank· DDBJ
MBB3133986.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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