A0A7W4V0A5 · A0A7W4V0A5_9MICO

Function

function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per monomer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site140Zn2+ 1 (UniProtKB | ChEBI)
Binding site143Zn2+ 1 (UniProtKB | ChEBI)
Binding site157Zn2+ 2 (UniProtKB | ChEBI)
Binding site160Zn2+ 2 (UniProtKB | ChEBI)
Binding site183Zn2+ 2 (UniProtKB | ChEBI)
Binding site186Zn2+ 2 (UniProtKB | ChEBI)
Binding site197Zn2+ 1 (UniProtKB | ChEBI)
Binding site200Zn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionheat shock protein binding
Molecular Functionunfolded protein binding
Molecular Functionzinc ion binding
Biological Processchaperone cofactor-dependent protein refolding
Biological ProcessDNA replication
Biological Processprotein refolding
Biological Processresponse to heat

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Chaperone protein DnaJ

Gene names

    • Name
      dnaJ
    • ORF names
      FHX49_000059

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 27099
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Micrococcales > Microbacteriaceae > Microbacterium

Accessions

  • Primary accession
    A0A7W4V0A5

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain, zinc finger.

TypeIDPosition(s)Description
Domain3-67J
Domain127-209CR-type
Zinc finger127-209CR-type

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.

Sequence similarities

Belongs to the DnaJ family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    372
  • Mass (Da)
    39,918
  • Last updated
    2021-06-02 v1
  • Checksum
    7071A955299DABEF
MADHYEVLGVSREATSDEIKKAYRRLARQLHPDVNPGEDASEKFKLVTHAYDVLSDPEQRRRYDMGGDSSPFGGGGGNANFGGFGDIFETFFGGGGQTRGARARSRAERGQDALVRLDLELGDVVFGVHRDLEVDTAVLCQTCEGSCCQPGTSPVTCDICHGSGHVQRTVRSLLGNVVTNQPCATCQGYGTTIPYPCATCQGQGRVRERRTVSLDIPAGVENGLRLQLPGSGEVGPAGGPNGDLYIEVSVAPHETFSREGDDLLATIEVSMPDAILGTTTKIDSLDGSVELDLRAGVQSGDVLTIKGRGITPLRGSQRGDLRVGVQVVTPTRLDSKERQMIAEFAARTKAPAPRLAEFHQGLFSKLRDRFRS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACHWQ010000001
EMBL· GenBank· DDBJ
MBB2974518.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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