A0A7W1XEI8 · A0A7W1XEI8_9ENTE
- ProteinMultifunctional fusion protein
- GenepyrF
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids450 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).
Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
Catalytic activity
- orotidine 5'-phosphate + H+ = UMP + CO2
Cofactor
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 13 | substrate | |||
Binding site | 36 | substrate | |||
Active site | 63 | For OMPdecase activity | |||
Binding site | 63-72 | substrate | |||
Active site | 65 | For OMPdecase activity | |||
Active site | 65 | Proton donor | |||
Active site | 68 | For OMPdecase activity | |||
Binding site | 126 | substrate | |||
Binding site | 188 | substrate | |||
Binding site | 197 | substrate | |||
Binding site | 217 | substrate | |||
Binding site | 218 | substrate | |||
Binding site | 337 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 341 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 343 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 363-371 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 367 | orotate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | magnesium ion binding | |
Molecular Function | orotate phosphoribosyltransferase activity | |
Molecular Function | orotidine-5'-phosphate decarboxylase activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameOrotidine 5'-phosphate decarboxylase
- EC number
- Alternative names
- Recommended nameOrotate phosphoribosyltransferase
- EC number
- Short namesOPRT ; OPRTase
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Enterococcaceae > Enterococcus
Accessions
- Primary accessionA0A7W1XEI8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 7-233 | Orotidine 5'-phosphate decarboxylase | |||
Sequence similarities
Belongs to the OMP decarboxylase family. Type 1 subfamily.
Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.
In the C-terminal section; belongs to the OMP decarboxylase family.
In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length450
- Mass (Da)49,820
- Last updated2021-06-02 v1
- ChecksumFDB66F5D8C43701A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JACEIT010000002 EMBL· GenBank· DDBJ | MBA4545133.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAIFOD010000037 EMBL· GenBank· DDBJ | MBX4194280.1 EMBL· GenBank· DDBJ | Genomic DNA |