A0A7W1XEI8 · A0A7W1XEI8_9ENTE

  • Protein
    Multifunctional fusion protein
  • Gene
    pyrF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).
Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site13substrate
Binding site36substrate
Active site63For OMPdecase activity
Binding site63-72substrate
Active site65For OMPdecase activity
Active site65Proton donor
Active site68For OMPdecase activity
Binding site126substrate
Binding site188substrate
Binding site197substrate
Binding site217substrate
Binding site218substrate
Binding site3375-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site3415-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site3435-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site363-3715-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site367orotate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionmagnesium ion binding
Molecular Functionorotate phosphoribosyltransferase activity
Molecular Functionorotidine-5'-phosphate decarboxylase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Orotidine 5'-phosphate decarboxylase
  • EC number
  • Alternative names
    • OMP decarboxylase
      (OMPDCase
      ; OMPdecase
      )
  • Recommended name
    Orotate phosphoribosyltransferase
  • EC number
  • Short names
    OPRT
    ; OPRTase

Gene names

    • Name
      pyrF
    • Synonyms
      pyrE
    • ORF names
      H1Z91_02045
      , KYX84_08805

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • s-7
    • S-7
    • EF216
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Lactobacillales > Enterococcaceae > Enterococcus

Accessions

  • Primary accession
    A0A7W1XEI8

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain7-233Orotidine 5'-phosphate decarboxylase

Sequence similarities

Belongs to the OMP decarboxylase family. Type 1 subfamily.
In the C-terminal section; belongs to the OMP decarboxylase family.
In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    450
  • Mass (Da)
    49,820
  • Last updated
    2021-06-02 v1
  • Checksum
    FDB66F5D8C43701A
MNQLNQRPIIALDFSTWQEVEDFLRFFPEEEKLFVKIGMELFYQEGPEIVRYLKDAGHDVFLDLKLHDIPNTVEKAMRGLAKLGVDVTCVHAAGGIRMMEAAMRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVPLEKSVIHYANCAKKAGLDGVVSSAWEVEAIKETAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAREIGSTFIVVGRPITQATDPYEAYRTIQTEWSQPKMNVEQSIAKDLLEIEAVFLNPSDPFTWASGIKSPIYCDNRITMSYPKVRKEIAKGLASKIKEAFPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDHGKGNQIEGRIVEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGKANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACEIT010000002
EMBL· GenBank· DDBJ
MBA4545133.1
EMBL· GenBank· DDBJ
Genomic DNA
JAIFOD010000037
EMBL· GenBank· DDBJ
MBX4194280.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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