A0A7U8EY00 · A0A7U8EY00_BORBG

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Features

Showing features for binding site, active site, site.

135550100150200250300350
TypeIDPosition(s)Description
Binding site6-13ATP (UniProtKB | ChEBI)
Binding site33ATP (UniProtKB | ChEBI)
Active site100Nucleophile
Binding site123ATP (UniProtKB | ChEBI)
Site124Interaction with tRNA
Active site195Cysteine persulfide intermediate
Site334Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionsulfurtransferase activity
Molecular FunctiontRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
Molecular FunctiontRNA binding
Biological Processmethylation
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      trmU
    • Synonyms
      mnmA
    • ORF names
      BBU118A_0701

Organism names

  • Taxonomic identifier
  • Strain
    • 118a
  • Taxonomic lineage
    Bacteria > Spirochaetota > Spirochaetia > Spirochaetales > Borreliaceae > Borreliella

Accessions

  • Primary accession
    A0A7U8EY00

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region145-147Interaction with tRNA
Domain204-266tRNA-specific 2-thiouridylase MnmA-like central
Domain276-350tRNA-specific 2-thiouridylase MnmA-like C-terminal

Sequence similarities

Belongs to the MnmA/TRMU family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    355
  • Mass (Da)
    41,518
  • Last updated
    2021-06-02 v1
  • Checksum
    5BF3298F736CA37A
MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDELSYIGNCPWQEDLNYVEAICNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKINSQYDLVVTGHYAKIQRKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSEVRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYWFFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTNFENFKIKIRHGEKKYSCKLKLITNNLMEISLNKKDQGISPGQFAIFYKNTECLGGAKIFKIIE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ABGI02000006
EMBL· GenBank· DDBJ
EEG98947.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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