A0A7U8EY00 · A0A7U8EY00_BORBG
- ProteintRNA-specific 2-thiouridylase MnmA
- GenetrmU
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids355 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- uridine34 in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine34 in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H+
RHEA-COMP:11727 CHEBI:65315 Position: 34+ RHEA-COMP:11726 + CHEBI:17499 + CHEBI:30616 = RHEA-COMP:11728 CHEBI:87170 Position: 34+ RHEA-COMP:10131 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 6-13 | ATP (UniProtKB | ChEBI) | ||||
Sequence: LLSGGVDS | ||||||
Binding site | 33 | ATP (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Active site | 100 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 123 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 124 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 195 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 334 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | sulfurtransferase activity | |
Molecular Function | tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | methylation | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Spirochaetota > Spirochaetia > Spirochaetales > Borreliaceae > Borreliella
Accessions
- Primary accessionA0A7U8EY00
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 145-147 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Domain | 204-266 | tRNA-specific 2-thiouridylase MnmA-like central | ||||
Sequence: EFIKYHLGEKKGNIIEKETGKIIGIHNGYWFFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISH | ||||||
Domain | 276-350 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | ||||
Sequence: RKFLVHEIHWINDTPTNFENFKIKIRHGEKKYSCKLKLITNNLMEISLNKKDQGISPGQFAIFYKNTECLGGAKI |
Sequence similarities
Belongs to the MnmA/TRMU family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length355
- Mass (Da)41,518
- Last updated2021-06-02 v1
- Checksum5BF3298F736CA37A