A0A7U6KFW0 · A0A7U6KFW0_9FIRM
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids653 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 81-82 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 105 | N6-AMP-lysine intermediate | |||
Binding site | 126 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 160 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 298 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 387 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 390 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 407 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 412 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA repair | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Lachnospirales > Lachnospiraceae
Accessions
- Primary accessionA0A7U6KFW0
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length653
- Mass (Da)73,433
- Last updated2021-06-02 v1
- MD5 Checksum146115C815EC7C5AA5791509228A27D0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP018794 EMBL· GenBank· DDBJ | BBF43174.1 EMBL· GenBank· DDBJ | Genomic DNA |