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A0A7U6KFW0 · A0A7U6KFW0_9FIRM

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

165350100150200250300350400450500550600650
Type
IDPosition(s)Description
Binding site81-82NAD+ (UniProtKB | ChEBI)
Active site105N6-AMP-lysine intermediate
Binding site126NAD+ (UniProtKB | ChEBI)
Binding site160NAD+ (UniProtKB | ChEBI)
Binding site298NAD+ (UniProtKB | ChEBI)
Binding site387Zn2+ (UniProtKB | ChEBI)
Binding site390Zn2+ (UniProtKB | ChEBI)
Binding site407Zn2+ (UniProtKB | ChEBI)
Binding site412Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      lbkm_1860

Organism names

Accessions

  • Primary accession
    A0A7U6KFW0

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain572-653BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    653
  • Mass (Da)
    73,433
  • Last updated
    2021-06-02 v1
  • MD5 Checksum
    146115C815EC7C5AA5791509228A27D0
MDQVKMDRMKELVTLLNQAGKAYYQDASEIMSNFEYDKLYDELVALEKETNVTLSNSPTVHVGYEVLSELPKERHPSSMLSLDKTKDVDELQSFLGSQEALLSWKLDGLTVVLTYENGTLQKAVTRGNGEIGEVITNNAKVFKNVPINISYTGRLVLRGEAVIKYSDFEKINAMIEDVDAKYKNPRNLCSGSVRQLNNEITAKRNVHFFAFTLVQAEDVDFKNSREEQMKFLTSLGFDTVEYKRVNAGNIHDTVAWFAEKIVSNDVPSDGLVLMYDDIAYSRSLGTTAKFPRDGIAFKWQDEVKETTLREIEWSASRTGLINPVAIFDPVELEGTTVSRASVHNVSIVESLELGIGDKIDVYKANMIIPQIAENKTRSSKLEIPSHCPVCGGETQIKQDNDVKALYCINPDCLAKRIKSFTHFVSRDAMNIEGLSEATIEKFIAKGFIKELADLYHVGQHKEEIKVMEGFGDKSFDNLMKSLEKARHTSLAKFIYSLGIPNIGLSNAKLICKEYKDDFDAIRNANVQDLVDVQGIGEVIAQTFVSYFRKEENQKIVDDLLKEITFEKAAESNTEQIFDGMNFVITGSVEHYANRNEVKAVIEERGGKVTGSVTSKTNYLINNDYLSNSSKNKKAKELNIPIITEEEFIAMLNE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP018794
EMBL· GenBank· DDBJ
BBF43174.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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