A0A7U3V2L7 · A0A7U3V2L7_9GAMM

Function

function

Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds two Mg2+ per subunit. The active form of the enzyme binds two Mg2+ ions in its active site. The first Mg2+ forms only one salt bridge with the protein.

Features

Showing features for binding site, site, active site.

119520406080100120140160180
Type
IDPosition(s)Description
Binding site16Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site16Mg2+ 2 (UniProtKB | ChEBI); catalytic
Binding site18Mg2+ 2 (UniProtKB | ChEBI); catalytic
Site22Important for substrate binding and specificity
Site70Important for substrate binding and specificity
Site117Important for substrate binding and specificity
Site138Important for substrate binding and specificity
Active site173Proton donor/acceptor
Binding site173Mg2+ 2 (UniProtKB | ChEBI); catalytic
Binding site178Mg2+ 2 (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3'-5' exonuclease activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleic acid binding
Molecular FunctionRNA exonuclease activity, producing 5'-phosphomonoesters
Biological ProcessDNA replication proofreading
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease T
  • EC number
  • Alternative names
    • Exoribonuclease T
      (RNase T
      )

Gene names

    • Name
      rnt
    • ORF names
      CVPH_0678

Organism names

Accessions

  • Primary accession
    A0A7U3V2L7

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain11-195Exonuclease

Sequence similarities

Belongs to the RNase T family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    195
  • Mass (Da)
    22,043
  • Last updated
    2021-09-29 v1
  • MD5 Checksum
    0CEF9A9243436DBE51590A2D51A05FE8
MLIKDRIRGYLPVVIDIETGGFNEKTDAMLEICAIVIGVDKVGNLYPKEPQHFHIEPFKGSILEPSALKFNGIDVDNPFRMAIKEVNAIKRIYTKVYTELKQENCTRAILIGHNAFFDLGFLRAATDRCKLKSPFHQFSTLDTVSLSALHYGETVLAKAMRKADIEWDDACAHSALYDTQKTAELFCKIFNTKPI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012977
EMBL· GenBank· DDBJ
BBB22714.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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