A0A7U3TI53 · A0A7U3TI53_DIOJA
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids685 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 69 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 71 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 87 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 90 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 492 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 494 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 496 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Dioscoreales > Dioscoreaceae > Dioscorea
Accessions
- Primary accessionA0A7U3TI53
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 265-546 | RNA polymerase N-terminal | ||||
Sequence: KRMVLCLLPVLPPELRPIIQIDGGKLMSSDINELYRRVIYRNNTLTDLLATSRSMPGELVMCQEKLVQEAVDTLLDNGIRGQPMKDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHQCGLPREIAIELFQTFLIHGLIKQHVASNIGIAKSKIREKEPIVWEILQEVMRGHPILLNRAPTLHRLGIQAFQPILVEERAICLHPLVCRGFNADFDGDQMAVHVPLSLEAQAEARLLMFSHMNLLSPAIGDPISVPTQDMLIGLYVLT |
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length685
- Mass (Da)79,085
- Last updated2021-06-02 v1
- Checksum4ACD24D8244017E2