A0A7U3NQV8 · A0A7U3NQV8_9STRA
- ProteinDNA-directed RNA polymerase subunit beta''
- GenerpoC2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1268 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 219 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 293 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 300 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 303 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta''
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Organism
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Ochrophyta > PX clade > Schizocladia
Accessions
- Primary accessionA0A7U3NQV8
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-61 | RNA polymerase Rpb1 | |||
Domain | 90-169 | RNA polymerase Rpb1 | |||
Domain | 171-1173 | RNA polymerase Rpb1 | |||
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,268
- Mass (Da)145,352
- Last updated2021-06-02 v1
- Checksum131E0738D149F30E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MT226925 EMBL· GenBank· DDBJ | QOW07524.1 EMBL· GenBank· DDBJ | Genomic DNA |