A0A7T5E027 · A0A7T5E027_9CYAN
- ProteinFumarate hydratase class II
- GenefumC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids465 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.
Miscellaneous
There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.
Catalytic activity
- (S)-malate = fumarate + H2O
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 101-103 | substrate | |||
Binding site | 132-135 | substrate; in site B | |||
Binding site | 142-144 | substrate | |||
Binding site | 190 | substrate | |||
Active site | 191 | Proton donor/acceptor | |||
Active site | 321 | ||||
Binding site | 322 | substrate | |||
Binding site | 327-329 | substrate | |||
Site | 334 | Important for catalytic activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | fumarate hydratase activity | |
Biological Process | fumarate metabolic process | |
Biological Process | malate metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFumarate hydratase class II
- EC number
- Short namesFumarase C
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Leptolyngbyales > Leptolyngbyaceae > Leptolyngbya group > Leptolyngbya
Accessions
- Primary accessionA0A7T5E027
Proteomes
Subcellular Location
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 15-345 | Fumarate lyase N-terminal | |||
Domain | 411-464 | Fumarase C C-terminal | |||
Sequence similarities
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length465
- Mass (Da)50,213
- Last updated2021-09-29 v1
- MD5 Checksum0475C484DCC107F67277A7FFB45BEFDD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP046155 EMBL· GenBank· DDBJ | QQE63540.1 EMBL· GenBank· DDBJ | Genomic DNA |