A0A7T4UQQ3 · A0A7T4UQQ3_9GAMM

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site184-185ATP (UniProtKB | ChEBI)
Binding site266ATP (UniProtKB | ChEBI)
Binding site288ATP (UniProtKB | ChEBI)
Binding site297ATP (UniProtKB | ChEBI)
Active site456Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular Functionsulfurtransferase activity
Molecular FunctiontRNA binding
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA 4-thiouridine biosynthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      I6N98_02420

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • csc3.9
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Cellvibrionales > Spongiibacteraceae > Spongiibacter

Accessions

  • Primary accession
    A0A7T4UQQ3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain62-166THUMP
Domain404-480Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    483
  • Mass (Da)
    53,970
  • Last updated
    2021-09-29 v1
  • Checksum
    CDAFD8EE03415C08
MKFVVKLFPEIIIKSKPVRRKFIKQLRDNLRKLLLPIDDAIQVERDWDRITVTSDADSELLPDIVAVLSNTPGIAHIEEVADYPFDDLHDIYEKTRAAVGDSLEGKRFAVRCKRTGTHDFNSSEVERYVGGGLNQHTGAAGVDLSKPEVVVRIEVRGDRLFVVRRRYEGLGGFPIGSVDSVMSLISGGFDSTVSSYLTMKRGLRTHFVFFNLGGREHEIGVKEVALYLWLKYGASHRVKFVSVPFEDVVAEILKNVENSQMGVILKRMMLRAASQVAEAMEAPALVTGESVAQVSSQTLANLSVIDSVTNTLVLRPLITMDKQDIIHTAAKIGTEEFAANMPEYCGVISVKPTTRAKPEKIAAQEANFDFAILEQAVANAEYLNIDELADEDRSLPEVEVLPIPLAEAVILDVRHPNEVDRHPLKLANGEVQSLPFYELHQRFEQLDQSRTYQLYCDKGVMSRLHAAHLVEQGFTNVKVYRPS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP066167
EMBL· GenBank· DDBJ
QQD18742.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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