A0A7S7PNC7 · A0A7S7PNC7_PVMA

  • Protein
    Genome polyprotein
  • Gene
    PVAgp1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Has helicase activity. It may be involved in replication.
Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
Mediates the cap-independent, EIF4E-dependent translation of viral genomic RNAs. Binds to the cap-binding site of host EIF4E and thus interferes with the host EIF4E-dependent mRNA export and translation. VPg-RNA directly binds EIF4E and is a template for transcription. Also forms trimeric complexes with EIF4E-EIF4G, which are templates for translation.

Catalytic activity

  • Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
    EC:3.4.22.45 (UniProtKB | ENZYME | Rhea)
  • Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
    EC:3.4.22.44 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site641For helper component proteinase activity
Active site714For helper component proteinase activity

GO annotations

AspectTerm
Cellular Componenthelical viral capsid
Cellular Componenthost cell cytoplasmic vesicle
Cellular Componenthost cell nucleus
Cellular Componentvesicle
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionhelicase activity
Molecular Functionhydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionserine-type peptidase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA-templated transcription
Biological Processproteolysis
Biological ProcessRNA-protein covalent cross-linking
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Genome polyprotein

Gene names

    • Name
      PVAgp1

Organism names

Accessions

  • Primary accession
    A0A7S7PNC7

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain154-298Peptidase S30
Domain633-755Peptidase C6
Domain1226-1378Helicase ATP-binding
Domain1397-1556Helicase C-terminal
Domain2032-2250Peptidase C4
Domain2516-2640RdRp catalytic
Region2797-2816Disordered
Compositional bias2801-2816Basic and acidic residues

Sequence similarities

Belongs to the potyviridae genome polyprotein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    3,059
  • Mass (Da)
    346,058
  • Last updated
    2021-06-02 v1
  • Checksum
    32CC4295E742C51C
MATQAIMVGEFKILEVNCKPHAPVAAIHVPTQAPKTNDIQWADLEFTLAKSLQRQAHGVVKVDKHGTARIKRASKHHMSCLEQQMADEVAEREAFMAAPTQLVTSIIFAGTTPPSMMETETIVKKIHTVGKHAKVMRKRSYITPXTDKSLRNHGVXPYSVQQLCRTLGNLSKRTGISLEVVGKTSKATKLRFTKTSFGYMARVQLKHHDGRMHRRDLVVDTSTTTIMQTLFLKTARTNANLDVLTHGSSGLVFWNYLVTGQRMRTRDNFIIVRGRCNGILVDARAKLSESTMLSTHHYSTGDVFWRGFNRTFLENKPINLDHVCSSDFSVEECGSIAALICQSLLPCGKITCRACAAKNLNMDEDTFKEFQTQRAREISAVIISKYPNFACVSQFIDRYFSHQRVLNPNVNAYREILKIVGGFTQSPYTHIQELNEILVLGGRATPEQLSSASAHLLEITRFVRNRTDNIKKGSLALFRNKISAKAHVNTALMCDNQLDRNGNLIWGERGYHAKRFFSNYFDIITPGGGYKQYIERRVPNGIRKLAIGNLIVTTNLEALREQLEGESIEKKAVTKACVSMSDNNYKYPCCCVTLDDGTPLYSTFIMPTKNHLVIGNSGDPKFLDLPTDISTQMYIAKSGYCYINIFLAMLVNVDESDAKDFTKKVRDIIVPDLGEWPTLIDVATSCSLLSAFYPATSAAELPRILVDHDLKTMHVIDSYGSLNTGYHVLKANTVRQLIQFASNSLDSEMKHYRVGGTPNSQINGYATIKMLAKAVYRPKLMKEIIHEQPYMLVMSLMSPGILIALANSGALEMGIHHWIREGDSLVKMAHMLRTVAQNVSVARATWVQQEIISDSAQQMLETILNGTIPNVSYFQAIQYLTMLAASKEVDAEVRVTGYYTFKLQTSELLEKTYLSLLEDSWQELSYFGRFQAIRHSRRYCTAGTIVVKPERHVDLGGIYATSYQFALAKQMEYSKKAVCQAVNGLQARFNNITSQIYCKILNWPKRLFPDLVKFINTMLAITVALQLYIAFATILRHHQQCKQDSLELEYCKKERQLITLYDFFIAKQPYATEEEFMAHVDEQNPDLSNFAREYCAEVVLFQAKASEQVNFERIIAFISLVLMMFDCERSDCVYRSLTKLKSLMSTVENTVQFQSLDDIGPTLEEKNMTIDFDLDTDTIVGKSIIGHTFKEWWDVQLNTNRIVPHYRTEGHFMEFTRANAPTIAHQISHDLHTDIMLRGAVGSGKSTGLPYHLSKKGTVLLLEPTRPLAENVTKQLKSDPFHVSPTLRMRGMAVFGSTPIHVMTTGFALHYLANNLKMLSTYDFIIIDEFHVHDSNAIALRNLLHEHNYQGKLIKVSATPPGREVEFSTQYPVEIRVEDQVSFQDFVKAQGNGSNLDLTSKCDNLLVYVASYNEVDQLSKLLLERHFLVTKVDGRSMKLGQVEIITKGSANKKHFIVATNIIENGVTLDIDAVIDFGMKVVPFLDSDNRMISYNKVSISYGERIQRLGRVGRNKAGVALRIGHTEKGISDVPVVIATQAAFLCFVYGLPISTQSVTTQVLSNVTLKQARTMVQFELPIFYMAHLVRYDGTMHPAIHNELKKYKLRDSEIQLSKLAIPSKCVPIWMTGKAYRLLTHNSQIPDDVRVPFLTKEIPDKLHENVWAIVEKFKCDAGIGRMTSAQASKVAYTLETDIHSIQRTILIIDQLLEREMQKQSHFEMVTNQSCSSGMLSLQTMMNAIQSRYAKNHTAGNIEILQRAKAQLLEFSNLSGDISTESALREFGYLEAVQFQSGTQMSNFLGLEGHWKKSLITKDLLIVGGVCVGAAWMIGEYFFKKSKEVVAFQGYNKRQRQKLKFARARDEKMGHYVEAPDSTLEHYFGSAYTKKGKTKGKTHGMGKKNHRFVNMYGFDPSDYTFIRYVDPLTGYTLDESPYTDIRLIQSQFSDIREQQLLNDELERNMVYHKPGVQGYLVKDKTSQILKIDLTPHIPLKVCDATNNIAGHPDREGELRQTGKGQLLDYAELPQKKELVEFESTSMFRGVRDYNPISSVICQLENESEGRTTQLFGLGFGPFIITNQHLFVRNNGSLTVRSQMGVFKVNSTVTLQMRPVEGRDVLIIKMPKDFPPFPQRLKFRQPTHSEKVCLILTNFQQKSSSSMVSETSHIIPKENTYFWKHWISTKEGHCGSPIVSTTDGAILGIHSLSNMTNTSNYFACFPKGFTETYLATESVHEWVKGWKFNASNVCWGSFHLQDSKPTKEFKTVKLVTDLLGEAVYTQGCDSKWLFNAAHTNIQAVAQLESNLVTKHTVKGKCKLFETYLNVDKAAHDFFSKYMGFYKPSKLNREAYTQDLMKYSKVIQVGEVDCEVFESALTGLLHNLGRWGFTTACYTTDEDSIYAALNMKAAVGALYRGKKRDYFDAMSPSEKEHLLFLSCKRLYFGQLGVWNGSLKAELRPKEKVDLNKTRTFTAAPIETLLGGKVCVDDFNNMFYSLHLKAPWSVGMTKFYGTWNQLMCKLPDDWVYCDADGSQFDSSISPYMINAVLRIRLHFMEDWDIGSQMLQNLYTEIVYTPISTPDGTVVKKFKGNNSGQPSTVVDNTLLVVLAMHYALLKSGVPLEEQDSVCVYCVNGDDLLIAIRPDMEHKLDGFQALFSELGLNYEFNSRSKDKKDLWFMSHKAIQCGEILIPKLEEERIVSILEWDRSHEPIHRLEAICASMVESWGYPELTHEIRRFYAWVLEQSPYNALATTGLAPYIAESALKTLYTNVHPTSTELEKYSIQFDEQMDEEDDMVYFQAGTLDAGETPAQKSEGKKKEGEGNSGKAVAVKDKDVDLGTAGTHSVPRLKSMTSKLTLPMLKGKSVVNLDHLLSYKPKQVDLSNARATHEQFQNWYDGVMASYELEESSMEIILNGFMVWCIENGTSPDINGVWTMMDNEEQVSYPLKPMLDHAKPSLRQIMRHFSALAEAYIEMRSREKPYMPRYGLQRNLRDQSLARYAFDFYEITATTPVRAKEAHLQMKAAALKNSNTNMFGLDGNVTTSEEDTERHTATDVNRNMHHLLGVKGV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias2801-2816Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MT521082
EMBL· GenBank· DDBJ
QOZ05775.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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