A0A7S5SRR8 · A0A7S5SRR8_9VIRU

Function

function

Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Catalytic activity

  • 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = L-aspartyl-[protein] + ADP-D-ribose + H+
    This reaction proceeds in the forward direction.
  • 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + ADP-D-ribose + H+
    This reaction proceeds in the forward direction.
  • ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.84 (UniProtKB | ENZYME | Rhea)
  • ATP + H2O = ADP + phosphate + H+
    EC:3.6.4.13 (UniProtKB | ENZYME | Rhea)
  • RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate
    EC:2.7.7.19 (UniProtKB | ENZYME | Rhea)
  • GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
  • N7-methyl-GTP + L-histidyl-[protein] = N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + diphosphate
    This reaction proceeds in the forward direction.
  • N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
  • a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H+
    This reaction proceeds in the forward direction.
    EC:3.6.1.74 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H+
    EC:3.6.1.15 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site1016For cysteine protease nsP2 activity
Active site1086For cysteine protease nsP2 activity

GO annotations

AspectTerm
Cellular Componentcytoplasmic vesicle membrane
Cellular Componenthost cell cytoplasmic vesicle membrane
Cellular Componenthost cell filopodium
Cellular Componenthost cell nucleus
Cellular Componenthost cell plasma membrane
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functioncysteine-type peptidase activity
Molecular FunctionGTP binding
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular FunctionmRNA methyltransferase activity
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Process7-methylguanosine mRNA capping
Biological ProcessDNA-templated transcription
Biological Processmethylation
Biological ProcessmRNA modification
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Polyprotein P1234
  • Alternative names
    • Non-structural polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • HaPV1/Phocoena phocoena/USA
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Togaviridae > Alphavirus

Accessions

  • Primary accession
    A0A7S5SRR8

Proteomes

Subcellular Location

Host cell membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Peripheral membrane protein
Host nucleus

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Interacts with RNA-directed RNA polymerase nsP4. Interacts with mRNA-capping enzyme nsP1. Interacts with KPNA1/karyopherin-alpha1; this interaction probably allows the active transport of protease nsP2 into the host nucleus.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain32-263Alphavirus-like MT
Domain679-994+RNA virus helicase C-terminal
Domain1007-1329Peptidase C9
Domain1325-1494Macro
Domain2243-2358RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,489
  • Mass (Da)
    278,474
  • Last updated
    2021-06-02 v1
  • Checksum
    6AE344D80B95E822
MEDKQQNVFIDTDPEGQFMKHVERCFPSFTVEARQVSANDHANARAFSHLAIKLIESEVPKDRVILDIGSAPARRMFAEHRYHCVCPMMTAEDPERMSRYVDRLKAESMLITNRNLANKMQDLRDVLATPALETESICLKTDSSCEMRGDVAVYQDVYAVHAPSSIYCQAIKGVRTVYWIGFDTTPFAFDALAGSYPLYSTNWADEKVLESRNIGLCASTLSEGKRRGWSICKSKKLKPSDNILFSVGSTLYHESRKMLKSWHLPAVFHLKGKESFTCRCETIVSCEGYVIKKISINPGIFGKASHYAVTHHYEGFLVAKVTDTISGVRTSFPVCTYVPAVICDQMTGILATDVTPEDAQKLLVGLNQRIVVNGRTQRNTNSIKNFLLPLIAQALSKWASEYKADLEGEGKMWERKRSISCCCLWAFQTKKQHTMYKRPDTQTIVKVPGEFNAFAPTPWSDGLPFSLRKKLLLLLRERTKRPPPITEEDVDTALNAEIESALVAEAEELVAALPPLMPTAPPMVDEIEVEELFERAGAGVVETPRNAIRVTAQDTDKVIGNYLIVSPQMVLRSKKLACVHQLAEEVKIMTHSGRTGRYAVEAYDGRVLLPVKSALKIPHFQALAESATMVYNEREFVNRKLYHIATRGAALNTDEEQYKCVKIEQTDHDFVFDVDAKSCKPRSMAKGLVLVGELTNPPYHEMAYESLNYRPSNVHDVEIVGVYGVPGSGKSGIIKQLVGPDDLVSSGKKENCLEIQRDVKQQRGLSIIARTVDSVLLNGVKRPVNVLFIDEAFACHAGTLLALIAIVKPKSKVILCGDPKQCGFFNMMQMKVNYNMEICDRVFNKSISRRCTQAVTAIVSTLHYDGKMRTVNPIIGNPVVDTTGSTKPKKGDLILTCFRGWVKQLQLDYRGYEVMTAAASQGLTRHGVYAVRQKVNENPLYALQSEHVNVLLTRTDNTLVWKTLSGDPWIKVLTSIPKGNFSATMQEWQREHDEILQALVTVPVYRDVFVGKTKVCWAKTLAPVLRTAGYTLTATEWGSLIPAFEDDEAFSPEVALNMICTRLFGHDLDSGLFSLEAVAMTYKDNHWDNSPGGKMWGFNEQVFNTLARRFPIMRQAIQHRQCALLQEHRIAPIEECPPIVPVNRELPHPLCTVILDRVRNNNSIYWKQLPGDTVMYIGMQAPNFGSKRVEWLGPLECGAASVKTDLSLGVPPTMGRYDGVFVDVPSRAMEHYKRCEEHAMKLRMIAGDSLRHLKPGAWMLVRTYGYADRISEVVLTTLLKKFRTYTVIQADLITCNAEVLILMKGFDNGNRNVSLARFSQATCGAFRGVQPAGSAPMYTVVRADIRTAETEAVVNAANPRGVPGDGVCGAVARRWPGSFVTGRIPVGECRAVRAEGTLILHAVGPDFRKVDEMSGGELLYKAYQACAKEVMKNGVKTVAVPLLSTGVYAGSKDRLEQSMMCLMDAFDATEANVHIYCRDKKWEERLKNIIKDKDTIEILLDEDVEVDTELVRVHPQSSLEGRPGYSATEGACYSFLLGTKFHQTAVDIAEIKALWPKDQEANEQICAYALGESMGLIREKCPVDDMGATAPPCTVPCLCPYAMTPERVFRVRAKAEQSLVVCSSFVLPRHAIKGVQRVPCRHLFVQERVEPVVKRVVNRSEAQGLRQRPLPTPPEENEEECLYMETYSEVGLNPSLGSSSWTLNMSEVRSPLLETVIEVATPSRFSIASSSVLQDSHQHVILADVHTPPTHLAECLTPPPVPPPRLKKLARMQAMKPPPKPPRTFAYDNHAFEACSLPNITFGSPEYVEVSFGLTTSELAQIANRANLCTSKISFGDFEAGEVESVCKKKRRRRRRRTRKSSSTQSLLPCSAXLERAGAYIFSSDTGQGHLQQRSVRQHDLTEVVLERVLPPAVFCPRYNQKKEETLLAIYQMNPTDANRSRYQSRKVENMKALTADRLIAGAKAFIKPSLLVECYKTMHAKIIYSSSVLRGLRSPIVAVRACNMFLSINYPTVCDYRITDEYDAYLDMVDGSESCLDRVSFCPAKLRYFPKQHSYHKPTIDSAVPSPFQNTLQNVLAAAMKRNCNVTQMRELPVYDSAVYNVECFKKYACNDEYWKEFSANPITLQNEDIRVYVAKLKGPKAAALFTKTTTLQRLEEVAMDRFVMDMKRDVKVVPGTKHTEERPKVQVIQAADAMATAYLCGIHRELVRRLKAVLRHNIHTLFDMSAEDFDAIIGEHFMPGDPVLETDIASFDKSQDDAMALTALMILEDLGVDTSLMDLIEVSFGEITSVHLPTGTRFRFGAMMKSGMFLTLFVNTILNVVIASRILEDRLTNSRCAAFIGDDNIIHGVVSDRLMADRCATWMNMEVKIIDAVMCERPPYFCGGFILWDCVTGLAGRVSDPLKRLFKLGKPLPADDTQDNDRCRAVRDEVRAWRRVGIQEDLQIAMESRYGITSSMLIIDALEAFSHNLDNFKEIRGPLVTLYGGPK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MN820528
EMBL· GenBank· DDBJ
QJE50387.1
EMBL· GenBank· DDBJ
Genomic RNA

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