A0A7S4QIE5 · A0A7S4QIE5_9DINO

  • Protein
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Non-allosteric.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site95diphosphate (UniProtKB | ChEBI)
Binding site189Mg2+ (UniProtKB | ChEBI); catalytic
Site190Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site216Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site217-219substrate
Active site219Proton acceptor
Binding site257-258substrate; ligand shared between dimeric partners
Binding site265-267substrate
Binding site326substrate
Binding site432-435substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processresponse to glucose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • EC number
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-dependent phosphofructokinase
      (PPi-PFK
      )
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • ORF names
      AMON00008_LOCUS20497

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • CCMP3105
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Dinophyceae > Gonyaulacales > Pyrocystaceae > Alexandrium

Accessions

  • Primary accession
    A0A7S4QIE5

Subcellular Location

Keywords

Interaction

Subunit

Homodimer or monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain87-457Phosphofructokinase
Domain662-908Phosphofructokinase

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,194
  • Mass (Da)
    129,709
  • Last updated
    2021-06-02 v1
  • Checksum
    7E4689B2BCBF2CB7
MAPNTQPQPYERARKAYSTLQRERLAADCRDYLPLVLEGCPKAQTKEAVPASAKDAEAIKGLFPSTYDTPAVEFAEGAGDGPRSPVKVGVVLSGGQAPGGHNVIAGIFDSVKEWHPDSTMIGFLDGPHGIFSGNFVEITGEIMDGFRNTGGFDMLGSGRHKIETEEQFQNSMAVCKAIDLDGLVVIGGDDSNTNGAVLAEYFKANGCKTRVVGAPKTIDGDLKCPPHIPVSFGFDTACKTFAALVGNVAVDALSAQKYYHLVRLMGRSASNIALEVALLTRPNVCLLGEEVSRDKKSLKDITVDLADMIEERSKMGKDYGVIVLPEGLIEFIPEFNSLISEINDKISSPDVQPTEEAVMAALSPENADSFRYLPPFIRAQLLLDRDPHGNVQVAKIETEKLLAATVVAELEERRKKGSYTGHFSPQFHSYGYEGRAGLPTVFDAAYCYALGATAANLLCSGLTGLIASVKNLLAPVKEWQCGGVPITSLCVIERRKGKDKPVIRKALVELEGDLSLPYQAYQAVRAELRLLDAYRVPGPMQFDMEKCPASRDIPLTLQLELGRPLPPVAKKPNIQKMGSFLYIPHSVESRSELQQWRIQRKHNLPKSLAEFRSNALEVHERASTMCFEHEQIIRKYFQRVNAPLVEIRATDAKQSAPAGQQVGVVFCGRQAPGCHDLVCGLVDMLHNSGNKVIGFVGGTQGLFTKQCVGLTPELCKAYAGTGGLELLGRTVDRIGSEEETAKVAKACQDLDLAGLVLVGGARTNTDAAYLAESFRSKGVKTAVVGVPCGIEGSMVNEFVEASLGFDSAAKTMAQLVGNTAVDGSSARKYYYFMKLMDGSATGGKLPTSHVALEAALQTKPNLLLLTEEVDEKRLSLREVVKEVADVVAQRAKDGKNFGTILVAEGLLAAIPEFRCLIQELEALPMPSPVEKVLPELTQWSRALFQSLPDFIQQQLLLERQSNKALQLSQLETERLVAWLVEDELRQRKRQGTYAGGFSPVCQFLGYQARCSMPSDFDSDYAYVLGGAAAVLAMNGYSGYMALVSDLSRPASEWRVGGAPLTAMLRVDHSPMQEARWARPTLFAARVDLEGPAFRQWCQVRAACAKDELYENPGPIQFCGPSASGVSTTIATKFSYMHELERLHKSIVEVSSRCRPGCDPRHVRVAVQSLATLNAILDELSVPLQAEAVSPEKTP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HBNR01030036
EMBL· GenBank· DDBJ
CAE4583728.1
EMBL· GenBank· DDBJ
Transcribed RNA

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