A0A7R6P5X0 · A0A7R6P5X0_9GAMM

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site41(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site41dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site41isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site74(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site74dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site74isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site96[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site124(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site124dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site124isopentenyl diphosphate (UniProtKB | ChEBI)
Active site126Proton donor
Binding site166(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site196[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site224(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site224dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site224isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site225(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site225dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site225isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site226(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site226dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site226isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site268(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site268dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site268isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      CVPH_1480

Organism names

Accessions

  • Primary accession
    A0A7R6P5X0

Proteomes

Family & Domains

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    308
  • Mass (Da)
    33,872
  • Last updated
    2021-09-29 v1
  • Checksum
    7F10BAA52F89FD07
MKILLANPRGFCAGVDRAIEIVERALKLHGAPVYVRHEVVHNKFVVDGLKAKGAVFVEDISDVPNNQVVIFSAHGVSMAVRKQTQNTGATIYDATCPLVAKVHKEVARKQKQNHQVILIGHKGHPEVEGTLGQSSEDKCVQLVETIEDVERLNLSKNTHISYTTQTTLSIDDTQHIVNYLKSKFPTIDAPKKDDICYATQNRQDGVKILMQSSDVLLVLGSSNSSNSNRLKEIAEKMGIPAYLIDGADEIDELWLKGMNTIGVTAGASAPEVLVQEVIHYLRDKGATKVIEVNGAKENIHFPVPKELR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012977
EMBL· GenBank· DDBJ
BBB23318.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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