A0A7N5JKH2 · A0A7N5JKH2_AILME

Function

function

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site58-77NAD+ (UniProtKB | ChEBI)
Binding site102substrate
Binding site105substrate
Binding site140-143NAD+ (UniProtKB | ChEBI)
Active site158Proton acceptor
Binding site166Zn2+ (UniProtKB | ChEBI)
Binding site169Zn2+ (UniProtKB | ChEBI)
Binding site181Zn2+ (UniProtKB | ChEBI)
Binding site194Zn2+ (UniProtKB | ChEBI)
Binding site231-233NAD+ (UniProtKB | ChEBI)
Binding site257-259NAD+ (UniProtKB | ChEBI)
Binding site275NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionNAD+ binding
Molecular FunctionNAD-dependent histone deacetylase activity
Molecular Functionprotein-malonyllysine demalonylase activity
Molecular Functionprotein-succinyllysine desuccinylase activity
Molecular Functiontransferase activity
Molecular Functionzinc ion binding
Biological Processprotein deacetylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • EC number
  • Alternative names
    • Regulatory protein SIR2 homolog 5
    • SIR2-like protein 5

Gene names

    • Name
      SIRT5

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Caniformia > Ursidae > Ailuropoda

Accessions

  • Primary accession
    A0A7N5JKH2

Proteomes

Subcellular Location

Mitochondrion
Cytoplasm, cytosol
Nucleus
Note: Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.

Keywords

Interaction

Subunit

Monomer. Homodimer. Interacts with CPS1.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain33-289Deacetylase sirtuin-type

Domain

In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity.

Sequence similarities

Belongs to the sirtuin family. Class III subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    292
  • Mass (Da)
    31,945
  • Last updated
    2021-06-02 v1
  • MD5 Checksum
    42617F99EA5361912FE6EB5D7E2131E3
MQPLQIAPRRLLYGLYCGLKSPASSGTQICPTMARPSSNMADFRKFFAKAKHVVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPQAFARNPSLVWEFYHYRREVMLSKEPNPGHLAIAECEARLHGQGRRVTVITQNIDELHRRAGTKNLLEIHGSLFKTRCTSCGIVAENYKSPICPALSGKGCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELTLCDLCLVVGTSSVVYPAAMFAPQVSARGVPVAEFNMETTPATNRFRFHFQGPCGTTLPEALAPHETENVS

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A7N5KH81A0A7N5KH81_AILMESIRT5310
G1L0W8G1L0W8_AILMESIRT5300
A0A7N5JHY9A0A7N5JHY9_AILMESIRT5315

Keywords

Genome annotation databases

Similar Proteins

Disclaimer

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