A0A7L5BVE9 · A0A7L5BVE9_9RHOB

  • Protein
    Bifunctional enzyme IspD/IspF
  • Gene
    ispDF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site15Transition state stabilizer
Site22Transition state stabilizer
Site152Positions MEP for the nucleophilic attack
Site206Positions MEP for the nucleophilic attack
Binding site232a divalent metal cation (UniProtKB | ChEBI)
Binding site232-2344-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site234a divalent metal cation (UniProtKB | ChEBI)
Site258Transition state stabilizer
Binding site258-2594-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site266a divalent metal cation (UniProtKB | ChEBI)
Binding site280-2824-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site356-3594-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site357Transition state stabilizer
Binding site3634-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site3664-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional enzyme IspD/IspF

Including 2 domains:

  • Recommended name
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • EC number
  • Alternative names
    • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    • MEP cytidylyltransferase
      (MCT
      )
  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispDF
    • ORF names
      G5B40_02855

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • RR4-56
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rhodobacterales > Paracoccaceae > Pikeienuella

Accessions

  • Primary accession
    A0A7L5BVE9

Proteomes

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-2252-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Domain225-3782-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region226-3812-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
Belongs to the IspF family.
In the C-terminal section; belongs to the IspF family.
In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    381
  • Mass (Da)
    40,169
  • Last updated
    2021-04-07 v1
  • Checksum
    6E1AC0E24C9AA6D9
MQIAALIVAAGRGSRSGPGVPKQYREIGGVPLLARTIRALLDSGRIHHVLTCIHPEDAELYRAVAPDDPRLSPPVRGGAERALSVRAGLEALALDPPDLVLIHDAARPFVSSHVIGDVIDALSLHDGAIAAVPVVDALRRSERGLCGAPVAREGVWRAQTPQGFRFGEILAAHRANTDHRAADDAEIARAAGLSVALVESAAENFKITTMRDFERAERQVGMEEIRIGQGYDVHAFTDGTAVTLCGVALPHDRALKGHSDADVAMHALTDAIFGAIAEGDIGRWFPPSEPEWKGAASSIFLEKAMARVRARGGRLVNADITIVCERPKIGPHAAAMTASLSRIMAVEAGRISVKATTSEGLGFVGREEGIAATALATVALP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP049056
EMBL· GenBank· DDBJ
QIE54467.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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