A0A7L4PS66 · A0A7L4PS66_9EURY

Function

function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site27Mg2+ 1 (UniProtKB | ChEBI)
Binding site80Mg2+ 1 (UniProtKB | ChEBI)
Binding site152Mg2+ 1 (UniProtKB | ChEBI)
Binding site154Mg2+ 1 (UniProtKB | ChEBI)
Binding site173Mg2+ 2 (UniProtKB | ChEBI)
Binding site175Mg2+ 2 (UniProtKB | ChEBI)
Binding site236Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function5'-3' exonuclease activity
Molecular Function5'-flap endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication, removal of RNA primer

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flap endonuclease 1
  • EC number
  • Short names
    FEN-1
  • Alternative names
    • Flap structure-specific endonuclease 1

Gene names

    • Name
      fen
    • ORF names
      GKC00_03775

Organism names

Accessions

  • Primary accession
    A0A7L4PS66

Proteomes

Interaction

Subunit

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-98N-domain
Domain1-101XPG N-terminal
Domain21-3255'-3' exonuclease
Domain140-221XPG-I
Region324-332Interaction with PCNA

Sequence similarities

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    332
  • Mass (Da)
    37,345
  • Last updated
    2021-04-07 v1
  • Checksum
    CD39834E4EC4BAC2
MGVNLGEIVPKKKIDISDLKGKSVAIDAYNALYQFLAIIRQRDGTPLKNSKGEVTSHLSGLFYRSVNFIEQGIRPIYVFDGKPPELKFQTIEKRKEIKEEAREKYIDAKSRGDFKEAKKYSQMTSSLKGDMITSSKELLTSMGIPYIEAPSEGEAQAAYVVLKGDADLSASQDYDSILFGAPALVRNLTISGKRKIPGKDLFVDVVPEIIDNQDVLNTLNLSRKQLIEVAILIGTDYNLGGIKGIGPKKAIEIVKEGKFGEYGEFDQIINLFLNPEVSDNYNISFEKPDKDRIVKILCDKYEFSELRVEKGIERIDFSLENTLKQKTLDMYF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAAEEM010000121
EMBL· GenBank· DDBJ
NYT03808.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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