A0A7K9XQH9 · A0A7K9XQH9_9GRUI
- ProteinCytosolic purine 5'-nucleotidase
- GeneNt5c2_1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids566 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine. Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP. Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency. Through these activities regulates the purine nucleoside/nucleotide pools within the cell.
Catalytic activity
- inosine + AMP = IMP + adenosine
- inosine + CMP = cytidine + IMP
- GMP + H2O = guanosine + phosphateThis reaction proceeds in the forward direction.
- inosine + GMP = guanosine + IMP
- XMP + H2O = xanthosine + phosphateThis reaction proceeds in the forward direction.
- dGMP + H2O = 2'-deoxyguanosine + phosphateThis reaction proceeds in the forward direction.
- dGMP + inosine = 2'-deoxyguanosine + IMP
- dIMP + H2O = 2'-deoxyinosine + phosphateThis reaction proceeds in the forward direction.
- dIMP + inosine = 2'-deoxyinosine + IMP
- inosine + UMP = uridine + IMP
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 52 | Nucleophile | |||
Binding site | 52 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 54 | Proton donor | |||
Binding site | 54 | GMP (UniProtKB | ChEBI) | |||
Binding site | 351 | Mg2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 5'-nucleotidase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | transferase activity | |
Biological Process | adenosine metabolic process | |
Biological Process | GMP metabolic process | |
Biological Process | IMP metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytosolic purine 5'-nucleotidase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Gruiformes > Psophiidae > Psophia
Accessions
- Primary accessionA0A7K9XQH9
Proteomes
Subcellular Location
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 530-566 | Disordered | |||
Compositional bias | 548-566 | Acidic residues | |||
Sequence similarities
Belongs to the 5'(3')-deoxyribonucleotidase family.
Family and domain databases
Sequence
- Sequence statusFragment
- Length566
- Mass (Da)65,546
- Last updated2021-04-07 v1
- Checksum2625878279FCB7A5
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-terminal residue | 1 | ||||
Compositional bias | 548-566 | Acidic residues | |||
Non-terminal residue | 566 | ||||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
VWZZ01006565 EMBL· GenBank· DDBJ | NXI99844.1 EMBL· GenBank· DDBJ | Genomic DNA |