A0A7K9X6B4 · A0A7K9X6B4_9GRUI

Function

function

The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.

Protein hedgehog N-product

The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development.

Protein hedgehog

The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site92Ca2+ 1 (UniProtKB | ChEBI)
Binding site93Ca2+ 1 (UniProtKB | ChEBI)
Binding site93Ca2+ 2 (UniProtKB | ChEBI)
Binding site98Ca2+ 1 (UniProtKB | ChEBI)
Binding site128Ca2+ 1 (UniProtKB | ChEBI)
Binding site129Ca2+ 2 (UniProtKB | ChEBI)
Binding site129Ca2+ 1 (UniProtKB | ChEBI)
Binding site132Ca2+ 2 (UniProtKB | ChEBI)
Binding site134Ca2+ 2 (UniProtKB | ChEBI)
Binding site143Zn2+ (UniProtKB | ChEBI)
Binding site150Zn2+ (UniProtKB | ChEBI)
Binding site185Zn2+ (UniProtKB | ChEBI)
Site200-201Cleavage; by autolysis
Site246Involved in cholesterol transfer
Site270Involved in auto-cleavage
Site273Essential for auto-cleavage

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentextracellular space
Cellular ComponentGolgi membrane
Cellular Componentplasma membrane
Molecular Functioncalcium ion binding
Molecular Functionpatched binding
Molecular Functionpeptidase activity
Molecular Functiontransferase activity
Biological Processanimal organ development
Biological Processcell development
Biological Processcell fate specification
Biological Processcell-cell signaling
Biological Processintein-mediated protein splicing
Biological Processneuron differentiation
Biological Processoligodendrocyte differentiation
Biological Processpattern specification process
Biological Processprotein autoprocessing
Biological Processregulation of cell population proliferation
Biological Processregulation of developmental process
Biological Processregulation of gene expression
Biological Processsmoothened signaling pathway
Biological Processtissue development
Biological Processtube development

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Hedgehog protein

Gene names

    • Name
      Shh
    • ORF names
      PSOCRE_R04203

Organism names

  • Taxonomic identifier
  • Strain
    • B10K-DU-001-60
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Gruiformes > Psophiidae > Psophia

Accessions

  • Primary accession
    A0A7K9X6B4

Proteomes

Subcellular Location

Endoplasmic reticulum membrane

Sonic hedgehog protein

Endoplasmic reticulum membrane
Golgi apparatus membrane

Protein hedgehog N-product

Cell membrane
; Lipid-anchor

Keywords

PTM/Processing

Features

Showing features for signal, chain.

Type
IDPosition(s)Description
Signal1-26
ChainPRO_502967599127-425Hedgehog protein

Keywords

Interaction

Subunit

Multimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain199-309Hint
Domain312-357Hint

Sequence similarities

Belongs to the hedgehog family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    425
  • Mass (Da)
    46,572
  • Last updated
    2021-04-07 v1
  • Checksum
    AF755EAA68F51E94
MDEMLLLTRILLVGFICALLVSSGLTCGPGRGIGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEEALHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLSPGDRVLAADAEGRLLYSDFLAFLDREDGSHKLFYVIETRQPRARLLLTAAHLVFVAPQHNQSQEGTPSSRALFASHIRPGQRVYVLGEGGQQLLPAAVHSVSLREEASGAYAPLTAQGTILINRVLASCYAVIEEHSWAHWAFAPFRLLQGMLAALCPSAGGVPVGAPADSGIHWYSRLLYRIGSWVLDSDSLHPLGMVVPSS

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue425

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VWZZ01002220
EMBL· GenBank· DDBJ
NXI92852.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp