A0A7K9T4I1 · A0A7K9T4I1_9PICI

  • Protein
    exodeoxyribonuclease III
  • Gene
    Apex1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Organism
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Probably binds two magnesium or manganese ions per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site14Mg2+ 1 (UniProtKB | ChEBI)
Active site89
Active site128Proton donor/acceptor
Binding site128Mg2+ 1 (UniProtKB | ChEBI)
Binding site130Mg2+ 1 (UniProtKB | ChEBI)
Site130Transition state stabilizer
Site159Important for catalytic activity
Binding site184Mg2+ 1 (UniProtKB | ChEBI)
Active site185Proton acceptor
Binding site185Mg2+ 1 (UniProtKB | ChEBI)
Site185Interaction with DNA substrate

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular FunctionDNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Functiondouble-stranded DNA 3'-5' DNA exonuclease activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular Functionmetal ion binding
Molecular Functionphosphoric diester hydrolase activity
Biological Processbase-excision repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    exodeoxyribonuclease III
  • EC number
  • Alternative names
    • APEX nuclease
    • Apurinic-apyrimidinic endonuclease 1

Gene names

    • Name
      Apex1
    • ORF names
      GALDEA_R12061

Organism names

  • Taxonomic identifier
  • Organism
    Galbula dea
  • Strain
    • B10K-DU-001-62
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Telluraves > Coraciimorphae > Piciformes > Galbulidae > Galbula

Accessions

  • Primary accession
    A0A7K9T4I1

Proteomes

Subcellular Location

Nucleus
Cytoplasm
Mitochondrion

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-185Endonuclease/exonuclease/phosphatase

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    194
  • Mass (Da)
    22,025
  • Last updated
    2021-04-07 v1
  • Checksum
    FBA4B13E2297DFF6
WVQEEAPDVLCLQETKCGAQAVPQELRDLPELPHQYWASPEDKPGYSGVGLLARHKPLNVTYGIGEEEHDREGRVVTAEFPSLYVVAAYVPNAGRRLVRLAYRQRWDQAFRSYLTRLDAHKPIALCGDLNVAYQDMPHAYTFWTYLGGARERNVGWRLDYLVISKRLEGALCDSKIRSQVMGSDHCPITLLLAL

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue194

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VWZX01006872
EMBL· GenBank· DDBJ
NXI42950.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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