A0A7K6Y0B9 · A0A7K6Y0B9_9PASE

Function

function

Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction. Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-phenylalanine = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-phenylalanine + H2O
    This reaction proceeds in the forward
    and the backward
    directions.
  • L-phenylalanine + (9Z)-octadecenoate = N-(9Z-octadecenoyl)-L-phenylalanine + H2O
    This reaction proceeds in the forward
    and the backward
    directions.
  • (9Z)-octadecenoate + glycine = N-(9Z-octadecenoyl)glycine + H2O
    This reaction proceeds in the backward direction.
  • N-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-L-phenylalanine + H2O = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + L-phenylalanine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine
    This reaction proceeds in the forward
    and the backward
    directions.
  • N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine
    This reaction proceeds in the forward
    and the backward
    directions.
  • N-(9Z-octadecenoyl)-L-asparagine + H2O = L-asparagine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-glutamine + H2O = L-glutamine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-leucine + H2O = L-leucine + (9Z)-octadecenoate
    This reaction proceeds in the forward
    and the backward
    directions.
  • N-(9Z-octadecenoyl)-L-lysine + H2O = L-lysine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-methionine + H2O = (9Z)-octadecenoate + L-methionine
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-serine + H2O = L-serine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-tryptophan + H2O = L-tryptophan + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-tyrosine + H2O = L-tyrosine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-hexadecanoyl-L-phenylalanine + H2O = hexadecanoate + L-phenylalanine
    This reaction proceeds in the forward direction.
  • N-octadecanoyl-L-phenylalanine + H2O = octadecanoate + L-phenylalanine
    This reaction proceeds in the forward direction.
  • an N-acyl-L-amino acid + H2O = an L-alpha-amino acid + a carboxylate
    This reaction proceeds in the forward
    and the backward
    directions.
    EC:3.5.1.14 (UniProtKB | ENZYME | Rhea)
  • an N-acyl-aromatic L-alpha-amino acid + H2O = an aromatic L-alpha-amino acid + a carboxylate
    This reaction proceeds in the forward
    and the backward
    directions.
    EC:3.5.1.114 (UniProtKB | ENZYME | Rhea)

Pathway

Amino-acid metabolism.
Lipid metabolism; fatty acid metabolism.

GO annotations

AspectTerm
Molecular Functionhydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
Molecular Functionmetal ion binding
Molecular Functionpeptidase activity
Biological Processamide biosynthetic process
Biological Processamide catabolic process
Biological Processamino acid metabolic process
Biological Processcellular lipid metabolic process
Biological Processproteolysis

Keywords

Names & Taxonomy

Protein names

  • Submitted names
    • P20D1 hydrolase

Gene names

    • Name
      Pm20d1
    • ORF names
      PROCAF_R08082

Organism names

  • Taxonomic identifier
  • Strain
    • B10K-UC-030-53
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Telluraves > Australaves > Passeriformes > Passeroidea > Nectariniidae > Promerops

Accessions

  • Primary accession
    A0A7K6Y0B9

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain250-393Peptidase M20 dimerisation

Sequence similarities

Belongs to the peptidase M20A family.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    514
  • Mass (Da)
    56,393
  • Last updated
    2021-04-07 v1
  • Checksum
    0C1AB7A25C248323
MAGGRRAVAAVLLALGSVVLALAAAVLLRAFVLRAPAGVPRLWARSAGTAALSAGERRELKEALRGAIKIPTVSRSPEDLNTTAMAEFGSYIQQVFPAVFSSKFIQHEIVGKYSHLFTVQGSAPGLLPYLLLAHMDVVPAPPEGWDFPPFSAAEHEGFIYGRGTLDNKNSAIGILQALEFLLRRNYRPRRSFYVGIGHDEEVSGLKGARRIAALLEARGVRLSFVLDEGSAVLDGIIAGVKKPVAMIAVTEKGSMTLNFTVEKEPGHSSFPPKETSIGILAAAMSRLEQNPLRNLFGYGPELMTMEHLASEFKFPLNIIMSNLWLFSPIISRVLAWKPSTNALIRTTTAVTLFNAGIKVNVIPSSARATVNFRIHSAETAAEVLEAVRNTIADDRVKIDVVEAFDPLPISPWDEQTFAIHIFQRTILDTFPDVDSVVPGTCIGNTDSRHFTNVTKAIYRFNPLLLKQDDLPRIHGLNERISIENYEKQVEFLFQLIKNCDIETLPEPHATSHEL

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue514

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VZSE01013028
EMBL· GenBank· DDBJ
NWX64856.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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