A0A7K3DKV4 · A0A7K3DKV4_9ACTN

Function

function

Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site993Mg2+ 1 (UniProtKB | ChEBI)
Binding site1000Mg2+ 2 (UniProtKB | ChEBI)
Binding site1000Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionlysine-tRNA ligase activity
Molecular Functionmagnesium ion binding
Molecular Functionphosphatidylglycerol lysyltransferase activity
Molecular FunctiontRNA binding
Biological Processlipid metabolic process
Biological Processlysyl-tRNA aminoacylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lysine--tRNA ligase
  • EC number
  • Alternative names
    • Lysyl-tRNA synthetase
      (LysRS
      )

Gene names

    • Name
      lysX
    • Synonyms
      lysS
    • ORF names
      GTW43_15690

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • SID5785
  • Taxonomic lineage
    Bacteria > Bacillati > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A7K3DKV4

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane20-38Helical
Transmembrane58-76Helical
Transmembrane83-103Helical
Transmembrane109-126Helical
Transmembrane146-166Helical

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain771-1074Aminoacyl-transfer RNA synthetases class-II family profile

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,081
  • Mass (Da)
    117,756
  • Last updated
    2021-04-07 v1
  • MD5 Checksum
    F97FE9AA4537C809185CE08331C5C12F
MTPPQSATARRWTHHVPRALSAAFAALAVYCALIAVIAPLRRLLRPVTRFLDALTVPVSANLAYAAFLLLLAGAAASRKKVAWWLVVVYLGLLIAVDAIGVGVGLYADALPSLIVCAIVVVVLLAARREFYAATRHGSLRRALTALLLGLAVSILVGWGLVALFPGSLRSGQHLLWASDKVCGGLVAAASYDGAPPRSLSFLLGLFGALALLNACATLFRSQRMEAALHGDEEPRIRALLGAYGSQDSLGYFATRRDKAVVFSPSGKAAVTYRVEAGVCLGSGDPVGDPEAWPHAIAAWLDIARRHAWSPAVMGASEEGATAFSRAGLGALQLGDEAVLHVADFDLDGRDMRVARQAVGRVRRTGATCRVRRHASLTEQEMEAIVDRADAWRDTETERGFSMALDRLGDPQDGDCLLVEALGADGELLALLSFVPWGRDGVSLDVMRRDRRAPNGVMEFMVAELCDVAGRYGIRRISLNFAVFRSVFEEGARIGAGPVLRLWRRLLLFLSRWWQLEALYRSNAKYRPAWQPRFICYADAGSLARISLASGIAEGFVSVPSLRRLWGKGHRPAAVTTPATTQGLPPIEELGLPSPDASPERQLPDQVRVRHRKLEALQAAGVDAYPVGLPARTHALGDITDATKGDQVTVAGRLMLVRDFGGIVFALLRDWSGDRQIALARDTAGQDTVAAFARGTDIGDHITASGQVGASDSGELTVFVTAWHLAAKCLRPLPDKRRGLADPETRVRRRYVDLIASREARDVVRARSTAVQALRQGLLSRGYLEVETPMLQQIHGGANARPFTTHINAYDLDLYLRIAPELYLKRLCVGGMEKVFEMGRTFRNEGVSYKHNPEFTMLEAYQAFADYDVMLDLTRELIQDAAVAACGEPVAYKDGVAHDISGPWPVKTVYGALSEALGEEVDADTALGRLLQLCERAQVPCTPDSGRGDVVLEMYERLVEEKTQLPTFYKDFPTDVSPLTRQHRADPRLAERWDLVAFGTELGTAYSELTDPVEQRRRLTAQSLLAAGGDPEAMELDEDFLDALEYAMPPTGGLGIGVDRLVMFLTGRTIRETLPFPLVRRR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WWIA01000475
EMBL· GenBank· DDBJ
MZD06528.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice.
Help