A0A7J7RNB4 · A0A7J7RNB4_RHIFE

Function

function

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Protein modification; protein glycosylation.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site104-106UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site135UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site178UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site183UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site208-210UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site210Mn2+ (UniProtKB | ChEBI)
Site241Interaction with galactose moiety of substrate glycoprotein
Active site297Proton donor/acceptor
Binding site324-326UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Site334Interaction with galactose moiety of substrate glycoprotein

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular ComponentGolgi membrane
Molecular Functiongalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
Molecular Functionmetal ion binding
Biological Processcarbohydrate metabolic process
Biological Processchondroitin sulfate proteoglycan biosynthetic process
Biological Processprotein glycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
  • EC number

Gene names

    • ORF names
      mRhiFer1_001324

Organism names

  • Taxonomic identifier
  • Strain
    • MRhiFer1
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Chiroptera > Yinpterochiroptera > Rhinolophoidea > Rhinolophidae > Rhinolophinae > Rhinolophus

Accessions

  • Primary accession
    A0A7J7RNB4

Proteomes

Subcellular Location

Golgi apparatus membrane
; Single-pass type II membrane protein
Secreted

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane20-38Helical

Keywords

PTM/Processing

Features

Showing features for glycosylation.

TypeIDPosition(s)Description
Glycosylation316N-linked (GlcNAc...) asparagine

Keywords

Interaction

Subunit

Homodimer. Interacts with SAR1A.

Family & Domains

Features

Showing features for region, compositional bias.

Type
IDPosition(s)Description
Region48-67Disordered
Compositional bias50-67Basic and acidic residues

Sequence similarities

Belongs to the glycosyltransferase 43 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    347
  • Mass (Da)
    39,769
  • Last updated
    2021-04-07 v1
  • Checksum
    90654C663AC48029
MGNEELWAQPVLEMPKRRDILAIVLIVLPWTLLVTVWHQSTIAPLLTAHKDDSSDPRRSTPLGTDPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRLANTLLHVPNLHWLVVEDAPRRTPLTAHLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPTVEI

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias50-67Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACAGC010000025
EMBL· GenBank· DDBJ
KAF6277652.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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