A0A7J7RJW4 · A0A7J7RJW4_MYOMY

Function

function

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site28Crucial for catalytic activity
Binding site94-107acetyl-CoA (UniProtKB | ChEBI)
Binding site130-139acetyl-CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentaxon
Cellular Componentclathrin-coated pit
Cellular Componentcytoplasm
Cellular Componentfocal adhesion
Cellular Componentmicrotubule
Cellular Componentspindle
Molecular Functiontubulin N-acetyltransferase activity
Biological Processalpha-tubulin acetylation
Biological Processneuron development
Biological Processregulation of microtubule cytoskeleton organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-tubulin N-acetyltransferase 1
  • EC number
  • Short names
    Alpha-TAT
    ; Alpha-TAT1
    ; TAT
  • Alternative names
    • Acetyltransferase mec-17 homolog

Gene names

    • Name
      ATAT1
    • Synonyms
      MEC17
    • ORF names
      mMyoMyo1_001127

Organism names

  • Taxonomic identifier
  • Strain
    • MMyoMyo1
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Chiroptera > Yangochiroptera > Vespertilionidae > Myotis

Accessions

  • Primary accession
    A0A7J7RJW4

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue26N6-acetyllysine; by autocatalysis
Modified residue116N6-acetyllysine; by autocatalysis

Post-translational modification

Autoacetylation strongly increases tubulin acetylation.

Keywords

Interaction

Subunit

Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-160N-acetyltransferase
Region199-230Disordered
Region253-279Disordered

Sequence similarities

Belongs to the acetyltransferase ATAT1 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    279
  • Mass (Da)
    31,605
  • Last updated
    2021-04-07 v1
  • Checksum
    26989BFB65B3B8C2
MTLLLSVDVQQQLMTIVDELGKASAKAQHLPAPITSASRMQSNRHVMYVLKDSSARPAGKGAIIGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHETLQRHGHGRELFQYMLQKERVEPHQLAIDRPSPKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHPPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPAARLLLATDPAASPAQRRRTSSLPRSEESRY

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A7J7RK67A0A7J7RK67_MYOMYATAT1420
A0A7J7RK45A0A7J7RK45_MYOMYATAT1331
A0A7J7RK48A0A7J7RK48_MYOMYATAT1309
A0A7J7RK56A0A7J7RK56_MYOMYATAT1398

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JABWUV010000026
EMBL· GenBank· DDBJ
KAF6276510.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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