A0A7J7M9K3 · A0A7J7M9K3_9MAGN
- ProteinAlpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids228 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Catalytic activity
- N4-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + UDP-N-acetyl-alpha-D-glucosamine = H+ + N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP
Cofactor
Note: The cofactor is mostly bound to the substrate.
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Cellular Component | nucleus | |
Molecular Function | alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity | |
Molecular Function | manganese ion binding | |
Biological Process | DNA topological change | |
Biological Process | protein glycosylation | |
Biological Process | resolution of meiotic recombination intermediates | |
Biological Process | sister chromatid segregation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- EC number
- Short namesGNT-I ; GlcNAc-T I
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Ranunculales > Circaeasteraceae > Kingdonia
Accessions
- Primary accessionA0A7J7M9K3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Membrane ; Single-pass type II membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 166-185 | Helical | ||||
Sequence: IRILLFAVAAAFIYIQMRLF |
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-223 | DNA topoisomerase type IIA | ||||
Sequence: MADLQRSIPSMVDGLKPGQRKILFCSFKQNFTKEAKVAQFSGYVLGHSAYHHGEQNLAGTIIGMAQVFVGSNNINLLQPNGHGNRHQGGKDHVSAWYVYTCLSPITRFLFPKDDDCLLDYLNEDGQSIEPIWYMPTIPMVLVNSSEEAFEGGFDPKETMTVKICDIRILLFAVAAAFIYIQMRLFRTQSGYADRLTAAMESESHCTSEMRLLIDQMSTPQERI |
Sequence similarities
Belongs to the glycosyltransferase 13 family.
Belongs to the type II topoisomerase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length228
- Mass (Da)25,566
- Last updated2021-04-07 v1
- Checksum69D5E63D9F43661D
Keywords
- Technical term