A0A7J7FB30 · A0A7J7FB30_DICBM

Function

function

A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Lipid metabolism; C21-steroid hormone metabolism.
Steroid metabolism; cholesterol metabolism.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site610Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmitochondrial inner membrane
Molecular Functioncholesterol monooxygenase (side-chain-cleaving) activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Biological ProcessC21-steroid hormone biosynthetic process
Biological Processcellular response to peptide hormone stimulus
Biological Processcholesterol metabolic process
Biological Processcortisol metabolic process
Biological Processglucocorticoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cholesterol side-chain cleavage enzyme, mitochondrial
  • EC number
  • Alternative names
    • Cholesterol desmolase

Gene names

    • ORF names
      HPG69_001707

Organism names

Accessions

  • Primary accession
    A0A7J7FB30

Proteomes

Subcellular Location

Membrane
; Peripheral membrane protein
Mitochondrion inner membrane
; Peripheral membrane protein
Note: Localizes to the matrix side of the mitochondrion inner membrane.

Keywords

Interaction

Subunit

Interacts with FDX1/adrenodoxin.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region19-40Disordered

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    669
  • Mass (Da)
    76,147
  • Last updated
    2021-04-07 v1
  • Checksum
    2ECC97BF5F6AD235
MAATSSWAPHYPRTQAVRTSSQCLPAAGSHSRDQQEAPAEAKAQTCIISVRSQRPAGAGAEDRDSGLSLCSGESLRCRNSRLSLGRGCAWAGASRRRREADCFISFSFSLELVVISWGPGAQGQSHPCCSGNRDSGSRGDNMLVRGLPLRSVLVKGCQPLLCAPREGLGHPRVPTGDGAGISSHSPRPFNEIPSPGDNGWLNLYHFWREKGSKKMHYHQLQNFQKYGPIYREKLGNLESVYIIDPEDVALLFKFEGPNPERYLIPPWVAYHEYYQKPIGVLFKKSRDWKKDRLALNQEVMAPESIKNFIPLLDAVSRDFVSILHRRIKQQGSGELSGDICEDLFRFAFESITNVIFGERLGTLEETVDPEAQQFIDAVYKMFHTSVPMLNFPPGLFRLLRTKTWRDHVASWDIIFSKAEKYTQNFYQDLKQKRDFSNYPGILYRLLGSNKLPFEDIKANVTEMLAGGVDTTSMTLQWHLYEMGRSLRVQEMLRKEVLAARRQAQGDMNMMVQLVPLLKASIKETLRQAHVHPCLLHPISVTLQRYITNDLVLRDYMIPAKTLVQASIYAMGQDPNFFLNPRCFDPTRWLDKNKDLVHFRNLGFGWGVRQCLGRRIAELEMTLFLIRILENFRVEIQHLNDVDTTFNLILTPDKPIYLTFRPLTQDPPQA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACDTQ010000812
EMBL· GenBank· DDBJ
KAF5925263.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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