A0A7J7F6B6 · A0A7J7F6B6_DICBM

Function

function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 + reduced [NADPH--hemoprotein reductase] = 21-hydroxy-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z)-eicosatrienoate + O2 + reduced [NADPH--hemoprotein reductase] = 19-hydroxy-(5Z,8Z,11Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH--hemoprotein reductase] = 19-hydroxy-(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • O2 + reduced [NADPH--hemoprotein reductase] + tetradecanoate = 13-hydroxytetradecanoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
    EC:1.14.14.1 (UniProtKB | ENZYME | Rhea)
  • an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • dodecanoate + O2 + reduced [NADPH--hemoprotein reductase] = 11-hydroxydodecanoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Activity regulation

The omega-1 hydroxylase activity is stimulated by cytochrome b5.

Pathway

Lipid metabolism; fatty acid metabolism.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site486Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentmitochondrial inner membrane
Molecular Functionaromatase activity
Molecular Functionheme binding
Molecular FunctionHsp70 protein binding
Molecular FunctionHsp90 protein binding
Molecular Functioniron ion binding
Biological Processfatty acid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • ORF names
      HPG69_010788

Organism names

Accessions

  • Primary accession
    A0A7J7F6B6

Proteomes

Subcellular Location

Endoplasmic reticulum membrane
; Peripheral membrane protein
Microsome membrane
; Peripheral membrane protein
Mitochondrion inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    542
  • Mass (Da)
    62,251
  • Last updated
    2021-04-07 v1
  • Checksum
    46CA99EF07E6CFD5
MAALGITVALLVWVATLLLVSIWKQIYSSWKLPPGPFPLPILGNVFQVDLKNIPKSFTRLAERYGPVFTLHLGSRRVVVLHGYKAVKEVLLTYKNEFSGRGEIPAFQEFKDKGVIFNNGPTWRDTRRFSLTVLRDYGMGKQRNEERIQRETHFLLEALRKTQGQPFDPTFVIGGGPFNVIADILFREHFDYEDKMCLRLMHLFNENFYLLSTPWLQVRPLSSVLVSLPSSSIAQFPLEKEGEFKSCGQTGLQELENRSQGVFDYPLYNYFSSYLRYLPGSHRKVMKNVSEIKAYTLERVKEHHKSLDSNRPRDFTDSLLVELEKEKHSAEPVFTLENIAVTTADMFFAGTETTSTTLRYGLLILLKYPEIEEKLHKEIDRVIGPSRIPAIKDRLEMPYMDAVVHEIQRFINLVPSNLPHEATRDTEFRGYVIPKGTVLIPTLDSLLYDDQEFPDPEEFKPEHFLNESGKFKYSDHFKAFSAGKRVCVGEGLARMELFLFLSAILQHFNLKSLVDPKDIDLSPITIGFGSIPPRYKLCVIPRS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACDTQ010001244
EMBL· GenBank· DDBJ
KAF5923569.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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