A0A7J7EYJ5 · A0A7J7EYJ5_DICBM

Function

function

NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site12-17FMN (UniProtKB | ChEBI)
Binding site59-62FMN (UniProtKB | ChEBI)
Binding site97-106FMN (UniProtKB | ChEBI)
Binding site132FMN (UniProtKB | ChEBI)
Binding site350FAD (UniProtKB | ChEBI)
Binding site382-385FAD (UniProtKB | ChEBI)
Binding site409-412FAD (UniProtKB | ChEBI)
Binding site460NADP+ (UniProtKB | ChEBI)
Binding site515-516NADP+ (UniProtKB | ChEBI)
Binding site521-525NADP+ (UniProtKB | ChEBI)
Binding site558NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmembrane
Cellular Componentperinuclear region of cytoplasm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular Functionmetal ion binding
Molecular FunctionNADP binding
Molecular FunctionNADPH-hemoprotein reductase activity
Biological Processiron-sulfur cluster assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent diflavin oxidoreductase 1
  • EC number
  • Alternative names
    • NADPH-dependent FMN and FAD-containing oxidoreductase

Gene names

    • Name
      NDOR1
    • ORF names
      HPG69_014807

Organism names

Accessions

  • Primary accession
    A0A7J7EYJ5

Proteomes

Subcellular Location

Cytoplasm, perinuclear region
Note: Concentrated in perinuclear structure.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane803-833Helical

Keywords

Interaction

Subunit

Interacts with CIAPIN1; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain6-150Flavodoxin-like
Domain206-447FAD-binding FR-type
Domain664-713RING-type
Region730-755Disordered
Compositional bias735-752Pro residues

Sequence similarities

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    838
  • Mass (Da)
    92,730
  • Last updated
    2021-04-07 v1
  • Checksum
    90B3E26540A91FF3
MPSPRLLVLFGSQTGTAQDVSDRLGREARRRRLGCLVQALDSYPVVNLINEPLVIFICATTGQGDPPDNMKNFWRFIFRKNLPSTSLCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLGGRALLPVCLGDDQHELGPDAAVDPWLHDLWEKVLGLYPVPLHLGIIPPGVPLPSKFTLHFLQEAPSTCSEEQHVTRTDPQGPPSELQPFLAPMVTNQRVTGPLHFQDVRLIEFDITGSGLSFAAGDVVLIQPENAAGHVQQFCQVLGLDPDQYFTLQPQEPGVPRPARLPQPCSVRHLVSRYLDIASVPRRSFFELLACLSPHELEREKLLEFSSAQGQEELCEYCSRPRRTILEVLGDFPHTAGAIPPDYLLDLIPLMRPRAFSIASSLLILVAVVQYQTRLKEPRRGLCSSWLASLDPRQGDPGTLAGPVQVPLWVRPGGLTFPETLDTPVIMVGPGTGVAPFRAALQERVAQGQTGNFLFFGCRRRDQDFYWEPEWKELEKRGCLTLVTAFSREQEQKVYVQHRLREFGPLVWELLDRQGAYFYLAGNAKYMPADVSEALTSIFQEEGGLSGPEAAAYLARLQRTLRFQTETPPSASLGPEGLDPLCQVLADEGLDSVALGNPQHARTPESEQLLGATAPACPWARPVGEDEEEEQGEEECPVCTEPYGPGEHRLALLNCGHGLCVGCLHRLLGTAPSADLGRVRCPLCRQKTPMLEWEICRLQEEXXXXDGPQRPPPPTPPTPPRRGPGPWASLEHRYQLRFLAGPVGGRGFLPFLPCPPCLGARLWALRGRGPCARRLALLGLLALELLGLLLIFTPLVLLGLLFMLLDRSSR

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias735-752Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACDTQ010001907
EMBL· GenBank· DDBJ
KAF5920777.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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