A0A7J7EIJ2 · A0A7J7EIJ2_DICBM

Function

function

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for site, binding site.

Type
IDPosition(s)Description
Site58Crucial for catalytic activity
Binding site124-137acetyl-CoA (UniProtKB | ChEBI)
Binding site160-169acetyl-CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentaxon
Cellular Componentclathrin-coated pit
Cellular Componentcytoplasm
Cellular Componentfocal adhesion
Cellular Componentmicrotubule
Cellular Componentspindle
Molecular Functiontubulin N-acetyltransferase activity
Biological Processalpha-tubulin acetylation
Biological Processneuron development
Biological Processregulation of microtubule cytoskeleton organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-tubulin N-acetyltransferase 1
  • EC number
  • Short names
    Alpha-TAT
    ; Alpha-TAT1
    ; TAT
  • Alternative names
    • Acetyltransferase mec-17 homolog

Gene names

    • Name
      ATAT1
    • Synonyms
      MEC17
    • ORF names
      HPG69_012653

Organism names

Accessions

  • Primary accession
    A0A7J7EIJ2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue56N6-acetyllysine; by autocatalysis
Modified residue146N6-acetyllysine; by autocatalysis

Post-translational modification

Autoacetylation strongly increases tubulin acetylation.

Keywords

Interaction

Subunit

Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain1-190N-acetyltransferase
Region229-261Disordered
Compositional bias232-247Pro residues
Region281-379Disordered
Compositional bias314-333Polar residues
Compositional bias334-353Basic and acidic residues
Compositional bias354-372Polar residues

Sequence similarities

Belongs to the acetyltransferase ATAT1 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    398
  • Mass (Da)
    44,732
  • Last updated
    2021-04-07 v1
  • Checksum
    E54FD41A2EA43115
MEFPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPITSASRMQSNRHVMYVLKDTSARPAGKGAIIGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHPPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLATDPGGSPAQRRRTRGSPPGLVAQSCCYSRHGGLNSSFPNTGNQESKQGEQETENRSASEEHVLSQDGSGEEPTHTVPPQAQAPPAQSWTMGGDMFNARFIRNLQERRSTRPW

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias232-247Pro residues
Compositional bias314-333Polar residues
Compositional bias334-353Basic and acidic residues
Compositional bias354-372Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JACDTQ010002860
EMBL· GenBank· DDBJ
KAF5915493.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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