A0A7J6KD88 · BPK1_TOXGO

  • Protein
    Bradyzoite pseudokinase 1
  • Gene
    BPK1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Required for the growth, maintenance, and/or stability, and thus infectivity, of bradyzoite cysts.

Caution

Although it belongs to the protein kinase superfamily, lacks the active site residue Asp at position 227 and the Asp residue at position 245 involved in Mg2+ chelation, suggesting that it has no protein kinase activity.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcyst wall
Cellular Componentextracellular space
Molecular FunctionATP binding
Molecular Functionprotein kinase activity
Biological Processprotein phosphorylation

Names & Taxonomy

Protein names

  • Recommended name
    Bradyzoite pseudokinase 1
  • Alternative names
    • Inactive serine/threonine-protein kinase BPK1

Gene names

    • Name
      BPK1
    • ORF names
      TGRH88_004990

Organism names

  • Taxonomic identifier
  • Strain
    • RH-88
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Apicomplexa > Conoidasida > Coccidia > Eucoccidiorida > Eimeriorina > Sarcocystidae > Toxoplasma

Accessions

  • Primary accession
    A0A7J6KD88
  • Secondary accessions
    • A0A452CSY6

Proteomes

Organism-specific databases

Subcellular Location

Secreted
Note: During the bradyzoite stage, secreted into the cyst lumen and localizes to the cyst wall.

Keywords

Phenotypes & Variants

Disruption phenotype

In a mouse infection model, tachyzoite growth, conversion to bradyzoites and cyst formation are normal 4 weeks post-infection (PubMed:23291621).
However, cysts are smaller and their capacity to cause oral infection in a mouse host is reduced which is probably due to their increased susceptibility to the conditions in the host digestive system (PubMed:23291621).

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-26
ChainPRO_502962114627-377Bradyzoite pseudokinase 1

Expression

Developmental stage

Expressed in tachyzoites and bradyzoites; expression increases following the tachyzoite to bradyzoite conversion (at protein level).

Interaction

Subunit

Forms a complex composed of BPK1, MCP4, MAG1, GRA8 and GRA9 (PubMed:23291621).
Interacts with MCP4 (PubMed:23291621).
Interacts with MAG1 (PubMed:23291621).
Interacts with GRA8 (PubMed:23291621).
Interacts with GRA9 (PubMed:23291621).

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region39-58Disordered
Domain48-354Protein kinase

Domain

The protein kinase domain is predicted to be catalytically inactive.

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    377
  • Mass (Da)
    42,203
  • Last updated
    2021-04-07 v1
  • Checksum
    EEF70B974FD5C666
MANTSVRRRQLLSSVLLLQWLTTVLGVAWGPNPIDGSKHGQFPSLRRTEGVSQSGSGHKGSVYRIPLADMTFWSWMSYLKELPDIDESRNPILKRLLSGSFLRRDGSTTVNVRVPARELVRLLSLTPEQQREGVSAKVRLINLLDPKYSVYEPYLYREILPKRSPLLLPSLGEYRGAFLTYIFHPLSKGLVGDMLETGRSHPDVQVLAANMVAALKSLHNLGLLHRSIELNSFSVLPDGTVVLGGLDTAAPIGTETRLWVGFFGQETAPEIDRNFLADLALTQGRHTVKSDVYSLGVAFRNLVQLLGNGNIGPEDRGAVRQDHLELLDKLSQKMIEEEPGNRPTIEEIMKDPLFEGLNFEDIEEGKARPFRYKQNRL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict311in Ref. 4

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAAUHK010000188
EMBL· GenBank· DDBJ
KAF4645395.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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