A0A7I8DJX8 · A0A7I8DJX8_9FIRM
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1242 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 69 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 71 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 84 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 87 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 458 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 460 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 462 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 804 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 887 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 894 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 897 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Lachnospirales > Lachnospiraceae > Anaerocolumna
Accessions
- Primary accessionA0A7I8DJX8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain, coiled coil, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 233-512 | RNA polymerase N-terminal | ||||
Sequence: DWMILTVVPVIPPDLRPMVQLDGGRFATSDMNDLYRRIINRNNRLKRLLELGAPDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKIYQCGLPKEMAIELFKPFVMKELVAKGTAHNIKSAKKMVERLQSEVWDVLEEVIREHPVMLNRAPTLHRLGIQAFEPVLVEGKAIKLHPLVCTAFNADFDGDQMAVHLPLSVEAQAECRFLLLSPNNLLKPSDGGPVAVPSQDMVLGIYYLT | ||||||
Coiled coil | 385-412 | |||||
Sequence: AKGTAHNIKSAKKMVERLQSEVWDVLEE | ||||||
Region | 1202-1242 | Disordered | ||||
Sequence: FNYSDDAMDDDLDSIDLDEFEDEDFSEEDFENDESLDDSDK | ||||||
Compositional bias | 1207-1242 | Acidic residues | ||||
Sequence: DAMDDDLDSIDLDEFEDEDFSEEDFENDESLDDSDK |
Sequence similarities
Belongs to the RNA polymerase beta' chain family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,242
- Mass (Da)138,871
- Last updated2021-04-07 v1
- ChecksumBEE7D141ED43A115
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1207-1242 | Acidic residues | ||||
Sequence: DAMDDDLDSIDLDEFEDEDFSEEDFENDESLDDSDK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP023368 EMBL· GenBank· DDBJ | BCJ97305.1 EMBL· GenBank· DDBJ | Genomic DNA |