A0A7G2C6Q3 · A0A7G2C6Q3_9TRYP
- ProteinSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids417 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity
- ATP + CoA + succinate = ADP + phosphate + succinyl-CoA
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 66 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 73-75 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GRG | ||||||
Binding site | 135 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 227 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 241 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 292 | substrate; ligand shared with subunit alpha | ||||
Sequence: N | ||||||
Binding site | 349-351 | substrate; ligand shared with subunit alpha | ||||
Sequence: GIM |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Strigomonadinae > Angomonas
Accessions
- Primary accessionA0A7G2C6Q3
Proteomes
Organism-specific databases
Subcellular Location
Interaction
Subunit
Heterodimer of an alpha and a beta subunit.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 22-230 | ATP-grasp fold succinyl-CoA synthetase-type | ||||
Sequence: NIHEYQSKRILKDNGGKVEFGIPCTTIEEVEAACANIKTPQKVVKSQILAGGRGKGHFKDGYQGGVKVCKDAKEAVEVAKKMLGNTLITKQTGAKGQVVNTLFVTEAVAGIKRELYVSLILDRKSASPIFIGSAEGGTGIEELAKTHPEKIKTMKVNISEGIDHDACVAYAKELGFTGDSAEKAANQFKVIYNIGKSKDCTMVEINPFI | ||||||
Domain | 290-407 | ATP-citrate synthase/succinyl-CoA ligase C-terminal | ||||
Sequence: LVNGAGLAMATMDLISLHGGSPANFLDVGGSAEEKQIVAAFRIITGDPNVKSILVNIFGGIMHCDVIAKGVVNAAKTLQTKVPIVVRLNGTNEEAGKQIIAESGMDVHTAEDFESGAK |
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length417
- Mass (Da)44,779
- Last updated2021-02-10 v1
- Checksum394B7EA3D4FC3513
Keywords
- Technical term