A0A7D9D1W2 · A0A7D9D1W2_9GAMM

Function

function

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

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TypeIDPosition(s)Description
Binding site189-192CoA (UniProtKB | ChEBI)
Binding site309CoA (UniProtKB | ChEBI)
Binding site333CoA (UniProtKB | ChEBI)
Binding site385-387ATP (UniProtKB | ChEBI)
Binding site409-414ATP (UniProtKB | ChEBI)
Binding site498ATP (UniProtKB | ChEBI)
Binding site513ATP (UniProtKB | ChEBI)
Binding site521CoA (UniProtKB | ChEBI)
Binding site524ATP (UniProtKB | ChEBI)
Binding site535Mg2+ (UniProtKB | ChEBI)
Binding site537Mg2+ (UniProtKB | ChEBI)
Binding site540Mg2+ (UniProtKB | ChEBI)
Binding site582CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionacetate-CoA ligase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Biological Processacetyl-CoA biosynthetic process from acetate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acetyl-coenzyme A synthetase
  • EC number
  • Short names
    AcCoA synthetase
    ; Acs
  • Alternative names
    • Acetate--CoA ligase
    • Acyl-activating enzyme

Gene names

    • Name
      acs
    • Synonyms
      acsA
    • ORF names
      JTBM06_V1_130008

Organism names

  • Taxonomic identifier
  • Strain
    • Gfbio:sag-sample-m06:053724c1-46a9-4a36-b237-ea2bf867836b
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Woeseiales > Woeseiaceae > environmental samples

Accessions

  • Primary accession
    A0A7D9D1W2

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue607N6-acetyllysine

Post-translational modification

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain22-79Acetyl-coenzyme A synthetase N-terminal
Domain81-465AMP-dependent synthetase/ligase
Domain529-607AMP-binding enzyme C-terminal

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    647
  • Mass (Da)
    71,818
  • Last updated
    2020-12-02 v1
  • Checksum
    915240EC5A9EABAD
MSKIHEVKAAFAKRAYVDQAGYEEMYRRSVEDNEGFWASQASRIDWIKPFSKVRDVSFAKDDLHIRWFYDGTLNVCYNCVDRHLEAKGDDVAIIWEGDDPDRDLTISYRELHQRVCRFANALIKIGAKKGDRITIYMPMIPETAVAMLACTRIGAVHSVVFGGFSPDALAGRIHDCESNIVITADQGLRGGKAIPLKANVDAAAEHADVTTLDKVLVVRNTGAEIAWVEGRDEWLHELEADVDADCPCAEMAAEDPLFILYTSGSTGKPKGVLHTTGGYIVYAAMTHQYVFDYHPGDIYWCTADVGWVTGHSYIVYGPLANGAITLMFEGLPNYPTASRFWEVCDKHQVNICYTAPTVIRALMREGDEPVKRTSRKSLRLLGTVGEPINPEAWEWYYEVVGEGRCPIVDTWWQTETGGILITALPGAMALKPGSASKPFFGIQPAVVDGDGNVLEGATDGNLVITDSWPGQMRSVYGDHQRFVETYFSAFEGKFFTGDGVRRDEDGYYWITGRVDDVLNVSGHRMGTAEIESALVAHKSVAEAAVVGCPHEIKGQGIYAYVTLMEGIEESDELLAELRQWVRKEIGPIATPDFIQWAPGLPKTRSGKIMRRILRKVAENDYSNLGDTSTLADPAVVEHLVENRLNRQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LR633967
EMBL· GenBank· DDBJ
VUX55831.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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