A0A7D7U4F8 · A0A7D7U4F8_9MOLU

  • Protein
    Thymidylate synthase
  • Gene
    thyA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site21dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site150-151dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site170
Active site170Nucleophile
Binding site191-194dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site194(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site202dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site232-234dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site288(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • ORF names
      H3143_00225

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 56A97T
  • Taxonomic lineage
    Bacteria > Mycoplasmatota > Mollicutes > Mycoplasmataceae > Mycoplasma

Accessions

  • Primary accession
    A0A7D7U4F8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-289Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    289
  • Mass (Da)
    33,718
  • Last updated
    2020-12-02 v1
  • Checksum
    3D041CDD8C82A9BE
MKTYLGLLEKVMTKGEVKKDRTNTGTISYFGTQGRYDLREGFPLLTTKKMAWKSICHELLWFLKGDTNIKYLVDHNVNIWNEWPYEAFKKSSDYNDETLQEFVDKIKADDQFAQVYGKLGPVYGKQWRNFNGVDQIEWVINEIKNNPFSRRLIVSAWNPSEIKDMALPPCHSFFQFFVNEKYEISLQLYQRSGDMFLGVPFNIASYSLLLLMVAQVTGLKPAEFVHTIGDTHIYSNHVDQVKIQLQREPKKLPKVSLNPDIKNIFDFKFEDISLENYEHHDPIKGKVAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP059674
EMBL· GenBank· DDBJ
QMT98570.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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