A0A6V8SGN7 · A0A6V8SGN7_9CLOT

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site13[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site42substrate
Binding site77substrate
Binding site99[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site127substrate
Active site129Proton donor
Binding site163substrate
Binding site191[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site219-221substrate
Binding site265substrate

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Molecular Functionnucleic acid binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      bsdtw1_01978

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • TW1
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium

Accessions

  • Primary accession
    A0A6V8SGN7

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain302-374S1 motif
Domain392-459S1 motif
Domain480-548S1 motif
Domain565-634S1 motif

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    636
  • Mass (Da)
    70,815
  • Last updated
    2020-12-02 v1
  • Checksum
    24D72975E534357F
MREILLAESAGFCFGVKRAVDETLKVQKQYNKKIFTIGPLIHNNDVVKMLENNNIFAIEINEIDSLANDDVVVIRSHGVPKSLVDKLNEKGVTVVNATCPYVTNIQKKVNKYHDEGYNIIIVGDKDHPEVVGINGWCNNEALVTKSGEFSDNLPEKICIVSQTTEKKSNWDKALKVVNEKSTDVLSFNTICSATDVRQRSAEEISKKVDAMIVIGGKNSSNTTKLFQICRSNCENTIHIENLEELPKELLNDNSIRTIGVTAGASTPDWIIKEVIDKMTMNNSEYNEQLELMEKSTKKIHVGEILKGEILSIVKNELIVAIDGYKADGVIPNSEISVNDGVKSKLSEHFKVGELVDAKVLRLQNEEGYVVLSRIELEKEKAFDETLEAYKNGSIVTVKVTEVVNGGVVATFKGLRVFIPASQLDIKYTGEFDNFVGKEIEVKFIEVEKERHIRIVASRRVLLEQEKHQREQDALNSLNVGDVVEGTVKRFTNFGAFVEVNGIDGLVHISEISWGKINNPSDVLKVGEKVKAKVIALDIEKKKVSLSVKALVQDPWMEIEEKYPEGSIVLGKVARLNDFGAFLELEPGVDGLAHISKISFNKISHPSEVLKVGELVKARIIKVEKENKRIGLSIKDV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BLZR01000001
EMBL· GenBank· DDBJ
GFP75886.1
EMBL· GenBank· DDBJ
Genomic DNA

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Disclaimer

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